PPP6R3 Protein (AA 1-844) (Strep Tag)

N° du produit ABIN3127694

Détail du produit

Antigène: PPP6R3 (Protein Phosphatase 6, Regulatory Subunit 3 (PPP6R3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-844

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PPP6R3 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MFWKFDLHSS SHIDTLLERE DVTLKELMDE EDVLQECKAQ NRKLIEFLLK AECLEDLVSF IIEEPPQDMD EKIRYKYPNI SCELLTSDVS QMNDRLGEDE SLLMKLYSFL LNESPLNPLL ASFFSKVLSI LISRKPEQIV DFLKKKRDFV DLIIKHIGTS AIMDLLLRLL TCIEPPQPRQ DVLNWLNEER IIQRLVEIVH PSQEEDRHSN ASQSLCEIVR LSRDQMLQVQ NSTEPDPLLA TLEKQEIIEQ LLSNIFHKEK NESAIVSAIQ ILLTLLETRR PTFEGHIEIC PPGMSHSACS VNKSVLEAIR GRLGSFHELL LEPPKKSVMK TTWGILDPPV GNTRLNVIRL ISSLLQTNTS SINGDLMELN SIGVILDMFF KYTWNNFLHT QVEICIALIL ASPFENAENG TITDQDSTGD NLLLKHLFQK CQLIERILEA WDTNEKKQAE GGRRHGYMGH LTRIANCIVH STDKGPNSAL VQQLIKDLPD EVRERWETFC TNSLGETNKR NTVDLAFSDY QMQQMTSNFI DQFGFNDEKF ADQDDIGNVS FDRVSDINFT LNTNESGNIA LFEACCKERI QQFDDGGSDE EDIWEEKHIA FTPESQRRSS SGSTDSEEST DSEEEDGAKQ DLFESSSANT EDKMEVDLNE PPTWSANFDV PMETTHGAPL DSVGSDVWST EEPMPTKETG WASFSEFTSS LSTKESLRSN SPVEMETSTE SVDPLTPGAA ALATQPEAPG SMAMEASSDG EEDAESTDKV TETVMNGGMK ETLSLTVDAK TETAVFKSEE EKLSTSQDAA CKDAEETPEP AEAKCTAPLT PSSSPEQRTD QPSMPSDPSV NGPV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PPP6R3 (Protein Phosphatase 6, Regulatory Subunit 3 (PPP6R3))

Autre désignation: Ppp6r3 (PPP6R3 Produits)

Synonymes: saps3 Protein, wu:fc31g07 Protein, zgc:56113 Protein, SAPS3 Protein, sapl Protein, pp6r3 Protein, sap190 Protein, MGC146363 Protein, DKFZp469M0915 Protein, C11orf23 Protein, PP6R3 Protein, SAP190 Protein, SAPL Protein, SAPLa Protein, 4930528G08Rik Protein, 9130026N02Rik Protein, D19Bwg1430e Protein, D19Ertd703e Protein, Pp6r3 Protein, Ppcs3 Protein, Pptcs3 Protein, Sapl Protein, Saps3 Protein, mKIAA1558 Protein, protein phosphatase 6, regulatory subunit 3 Protein, protein phosphatase 6 regulatory subunit 3 Protein, ppp6r3 Protein, PPP6R3 Protein, Ppp6r3 Protein

Sujet: Serine/threonine-protein phosphatase 6 regulatory subunit 3 (SAPS domain family member 3),FUNCTION: Regulatory subunit of protein phosphatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance (By similarity). {ECO:0000250}.

Poids moléculaire: 94.7 kDa

UniProt: Q922D4