ARL4D Protein (AA 2-201) (His tag)

N° du produit ABIN3128147

Détail du produit

Antigène: ARL4D (ADP-Ribosylation Factor-Like 4D (ARL4D))

Type de proteíne: Recombinant

Attributs du protein: AA 2-201

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette ARL4D protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: GNHLTEMAPT ASSFLPHFQA LHVVVIGLDS AGKTSLLYRL KFKEFVQSVP TKGFNTEKIR VPLGGSRGIT FQVWDVGGQE KLRPLWRSYT RRTDGLVFVV DSAETERLEE ARMELHRISK ASDNQGVPVL VLANKQDQPG ALSAAEVEKR LAVRELAAAT LTHVQGCSAV DGLGLQPGLE HLYEMILKRK KAPRSSKKRR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Arl4d Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARL4D (ADP-Ribosylation Factor-Like 4D (ARL4D))

Autre désignation: Arl4d (ARL4D Produits)

Synonymes: ARF4L Protein, ARL6 Protein, 1110036H21Rik Protein, AW456149 Protein, Arf4l Protein, Arfl4 Protein, Arl5 Protein, fc03d05 Protein, zgc:77741 Protein, wu:fc03d05 Protein, ARL4D Protein, ADP ribosylation factor like GTPase 4D Protein, ADP-ribosylation factor 4 Protein, ADP ribosylation factor 4 Protein, ADP-ribosylation factor-like 4D Protein, ADP-ribosylation factor like GTPase 4D Protein, ARL4D Protein, LOC709254 Protein, ARF4 Protein, Arl4d Protein, arl4d Protein, LOC100565035 Protein, LOC100565228 Protein, LOC100633115 Protein

Sujet: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form (By similarity). {ECO:0000250}.

Poids moléculaire: 23.1 kDa Including tag.

UniProt: Q99PE9