Necdin-Like 2 Protein (NDNL2) (AA 1-279) (Strep Tag)

N° du produit ABIN3128188

Détail du produit

Antigène: Necdin-Like 2 (NDNL2)

Type de proteíne: Recombinant

Attributs du protein: AA 1-279

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Necdin-Like 2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MLQKPRGRGR PSTQADPERD WGGAGEEGPS TSRAAGGSSQ GSRASLSAPT VGPRTQKQLE LKVAELVQFL LIKDQKKIPI KRTDILKHVV GDYRDVYPNL LKLAAERLQY VFGYKLVELE PKSHSYILIN MLEPVEADAE MRGDQGTPIS GLLMIVLGLI FMKGNTITET EVWDFLRRLG VYPTKKHLIF GDPKKLITED FVRQRYLEYR RIPHTDPVDY ELQWGPRTNL ETSKMKVLKF VAKVHNQDPK DWPTQYCEAL ADEESRARPA TASAPATSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Necdin-Like 2 (NDNL2)

Autre désignation: Nsmce3 (NDNL2 Produits)

Synonymes: mage Protein, zgc:101850 Protein, NDNL2 Protein, HCA4 Protein, MAGEG1 Protein, MAGEL3 Protein, NSE3 Protein, NSMCE3 Protein, 5730494G16Rik Protein, AI642138 Protein, BB044375 Protein, Mageg1 Protein, mage-g1 Protein, NSE3 homolog, SMC5-SMC6 complex component Protein, necdin-like 2 Protein, Nsmce3 Protein, ndnl2 Protein, NDNL2 Protein, NSMCE3 Protein

Sujet: Non-structural maintenance of chromosomes element 3 homolog (Non-SMC element 3 homolog) (MAGE-G1 antigen) (Necdin-like protein 2),FUNCTION: Component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). In vitro enhances ubiquitin ligase activity of NSMCE1. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (By similarity). May be a growth suppressor that facilitates the entry of the cell into cell cycle arrest (PubMed:14593116). {ECO:0000250|UniProtKB:Q96MG7, ECO:0000269|PubMed:14593116}.

Poids moléculaire: 31.5 kDa

UniProt: Q9CPR8