MED4 Protein (AA 2-270) (His tag)
-
- Antigène Voir toutes MED4 Protéines
- MED4 (Mediator Complex Subunit 4 (MED4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-270
-
Origine
- Souris
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette MED4 protéine est marqué à la His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Séquence
-
AASSSGEKEK ERMGGVSGMA GLGSTRERLL SALEDLEVLS RELIEMLAIS RNQKLLQLEE ENQVLELLIH RDGDFQELMK LALNQGKVHH EMQALEKEVE KRDSDIQQLQ KQLKEAEQIL ATAVYQAKEK LKSIEKARKG AISSEEIIKY AHRISASNAV CAPLTWVPGD PRRPYPTDLE MRSGLLGQMN NPSTSGVNGH LPGDALAAGR LPDVLAPQYP WQSNDMSVNM LPPNHSSDFL LEPPGHNKEN EDDVEVMSTD SSSSSSDSD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Med4 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product MED4 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- MED4 (Mediator Complex Subunit 4 (MED4))
- Autre désignation
- Med4 (MED4 Produits)
- Synonymes
- zgc:110646 Protein, ARC36 Protein, DRIP36 Protein, HSPC126 Protein, TRAP36 Protein, VDRIP Protein, Vdrip Protein, 2410046H15Rik Protein, Vdirp Protein, mediator complex subunit 4 Protein, mediator complex subunit 4 S homeolog Protein, med4 Protein, med4.S Protein, MED4 Protein, Med4 Protein
- Sujet
- Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). {ECO:0000250}.
- Poids moléculaire
- 30.6 kDa Including tag.
- UniProt
- Q9CQA5
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Regulation of Lipid Metabolism by PPARalpha
-