THEM2 Protein (AA 2-140) (His tag)

N° du produit ABIN3128354

Détail du produit

Antigène: THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))

Type de proteíne: Recombinant

Attributs du protein: AA 2-140

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette THEM2 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: SSMTQNLREV MKVMFKVPGF DRVLEKVTLV SAAPEKLICE MKVEEQHTNK LGTLHGGLTA TLVDSISTMA LMCTERGAPG VSVDMNITYM SPAKIGEEIV ITAHILKQGK TLAFASVDLT NKTTGKLIAQ GRHTKHLGN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Acot13 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: THEM2 (ACOT13) (Acyl-CoA Thioesterase 13 (ACOT13))

Autre désignation: Acot13 (ACOT13 Produits)

Synonymes: them2 Protein, MGC89869 Protein, si:dkey-218n20.4 Protein, THEM2 Protein, DKFZp468B074 Protein, PNAS-27 Protein, 0610006O17Rik Protein, Them2 Protein, acyl-CoA thioesterase 13 Protein, acyl-CoA thioesterase 13 S homeolog Protein, thioesterase superfamily member 2 Protein, Thioesterase superfamily protein Protein, acot13 Protein, ACOT13 Protein, acot13.S Protein, LOC733047 Protein, si605090c10 Protein, Acot13 Protein

Sujet: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis. {ECO:0000269|PubMed:19405909, ECO:0000269|PubMed:24072708}.

Poids moléculaire: 16.0 kDa Including tag.

UniProt: Q9CQR4