UQCRC1 Protein (AA 35-480) (His tag)

N° du produit ABIN3128686

Détail du produit

Antigène: UQCRC1 (Ubiquinol-Cytochrome C Reductase Core Protein I (UQCRC1))

Type de proteíne: Recombinant

Attributs du protein: AA 35-480

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette UQCRC1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: TATFAQALQS VPETQVSILD NGLRVASEQS SHATCTVGVW IDAGSRYETE KNNGAGYFLE HLAFKGTKNR PGNALEKEVE SIGAHLNAYS TREHTAYLIK ALSKDLPKVV ELLADIVQNS SLEDSQIEKE RDVILREMQE NDASMQNVVF DYLHATAFQG TPLAQAVEGP SENVRRLSRT DLTDYLNRHY KAPRMVLAAA GGVEHQQLLD LAQKHLSSVS RVYEEDAVPG LTPCRFTGSE IRHRDDALPL AHVAIAVEGP GWANPDNVTL QVANAIIGHY DCTYGGGVHL SSPLASVAVA NKLCQSFQTF NISYSDTGLL GAHFVCDAMS IDDMVFFLQG QWMRLCTSAT ESEVTRGKNI LRNALVSHLD GTTPVCEDIG RSLLTYGRRI PLAEWESRIQ EVDAQMLRDI CSKYFYDQCP AVAGYGPIEQ LPDYNRIRSG MFWLRF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Uqcrc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: UQCRC1 (Ubiquinol-Cytochrome C Reductase Core Protein I (UQCRC1))

Autre désignation: Uqcrc1 (UQCRC1 Produits)

Synonymes: MGC53748 Protein, zgc:73404 Protein, zgc:85750 Protein, 1110032G10Rik Protein, D3S3191 Protein, QCR1 Protein, UQCR1 Protein, ubiquinol-cytochrome c reductase core protein 1 L homeolog Protein, ubiquinol-cytochrome c reductase core protein 1 Protein, ubiquinol-cytochrome c reductase core protein I Protein, cytochrome b-c1 complex subunit 1, mitochondrial Protein, uqcrc1.L Protein, uqcrc1 Protein, UQCRC1 Protein, LOC100020379 Protein, LOC100074417 Protein, LOC100347571 Protein, LOC100413740 Protein, LOC100452425 Protein, Uqcrc1 Protein

Sujet: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1 (By similarity). {ECO:0000250}.

Poids moléculaire: 50.3 kDa Including tag.

UniProt: Q9CZ13