NT5C3 Protein (AA 1-331) (Strep Tag)

N° du produit ABIN3128759

Détail du produit

Antigène: NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-331

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NT5C3 protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MDRAAVARVG AVASASVCAV VAGVVLAQYI FTLKRKTGRK TKIIEMMPEF QKSSVRIKNP TRVEEIICGL IKGGAAKLQI ITDFDMTLSR FSYNGKRCPT CHNIIDNCKL VTDECRRKLL QLKEQYYAIE VDPVLTVEEK FPYMVEWYTK SHGLLIEQGI PKAKLKEIVA DSDVMLKEGY ENFFGKLQQH GIPVFIFSAG IGDVLEEVIR QAGVYHSNVK VVSNFMDFDE NGVLKGFKGE LIHVFNKHDG ALKNTDYFSQ LKDNSNIILL GDSQGDLRMA DGVANVEHIL KIGYLNDRVD ELLEKYMDSY DIVLVKEESL EVVNSILQKT L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NT5C3 (5'-Nucleotidase, Cytosolic III (NT5C3))

Autre désignation: Nt5c3a (NT5C3 Produits)

Synonymes: 1600024P05Rik Protein, 2610206B05Rik Protein, 3110004A18Rik Protein, AI842776 Protein, Nt5c3a Protein, Nt5y Protein, PN-1 Protein, PN-I Protein, PSN1 Protein, UMPH Protein, Umph-1 Protein, Umph1 Protein, cN-III Protein, lupin Protein, p36 Protein, fb30h07 Protein, nt5c3 Protein, wu:fb30h07 Protein, zgc:66117 Protein, NT5C3 Protein, PYN1 Protein, P5'N-1 Protein, P5N-1 Protein, POMP Protein, UMPH1 Protein, hUMP1 Protein, Nt5c3 Protein, 5'-nucleotidase, cytosolic III Protein, 5'-nucleotidase, cytosolic IIIA Protein, cytosolic 5'-nucleotidase 3A Protein, Nt5c3 Protein, nt5c3a Protein, NT5C3A Protein, LOC534181 Protein, Nt5c3a Protein, nt5c3a.L Protein, Bm1_56095 Protein

Sujet: Cytosolic 5'-nucleotidase 3A (EC 3.1.3.5) (7-methylguanosine phosphate-specific 5'-nucleotidase) (7-methylguanosine nucleotidase) (EC 3.1.3.91) (Cytosolic 5'-nucleotidase 3) (Cytosolic 5'-nucleotidase III) (cN-III) (Lupin) (Pyrimidine 5'-nucleotidase 1) (P5'N-1) (P5N-1) (PN-I),FUNCTION: Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate. {ECO:0000250|UniProtKB:Q9H0P0}.

Poids moléculaire: 37.3 kDa

UniProt: Q9D020