APITD1 Protein (AA 1-142) (His tag)
-
- Antigène Voir toutes APITD1 Protéines
- APITD1 (Apoptosis-Inducing, TAF9-Like Domain 1 (APITD1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-142
-
Origine
- Souris
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette APITD1 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
-
MEEVEAEEPQ EFSHRQRLKA AVHYTVGCLC QEVTLNKQVN FSKQTIAAIS EVTFRQCENF AKDLEMFARH AKRSTVTTED VKLLARRNNS LLKYITEKNE EIAQLNLKGK AKKKRKPEDE SRSSRESMAE ELDGAEELQS ES
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Apitd1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product APITD1 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- APITD1 (Apoptosis-Inducing, TAF9-Like Domain 1 (APITD1))
- Autre désignation
- Apitd1 (APITD1 Produits)
- Synonymes
- CENP-S Protein, CENPS Protein, FAAP16 Protein, MHF1 Protein, 2610040C18Rik Protein, 2810407L01Rik Protein, cenps Protein, mhf1 Protein, APITD1 Protein, Apitd1 Protein, Mhf1 Protein, apitd1 Protein, zgc:194319 Protein, centromere protein S Protein, apoptosis-inducing, TAF9-like domain 1 Protein, centromere protein S L homeolog Protein, CENPS Protein, Cenps Protein, cenps.L Protein, APITD1 Protein, cenps Protein
- Sujet
- DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Required for optimal chromatin association of the FA core complex. Required for efficient damage-induced monoubiquitination and focus formation of FANCD2. Stabilizes FAAD24, FANCM and APITD1/CENPS in the FA core complex. Plays a role in DNA interstrand cross-linking (ICL) repair and in recovery of replication forks stalled by topoisomerase I-DNA cleavage intermediates induced by camptothecin. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). {ECO:0000250}.
- Poids moléculaire
- 17.3 kDa Including tag.
- UniProt
- Q9D084
-