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MED27 Protein (AA 1-311) (His tag)

Crystallography grade MED27 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
N° du produit ABIN3129520
  • Antigène Voir toutes MED27 Protéines
    MED27 (Mediator Complex Subunit 27 (MED27))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-311
    Origine
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris
    Source
    • 4
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette MED27 protéine est marqué à la His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Séquence
    MADVLSVGVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIANFQDNL HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNES VYTEDGKLDI WSKSSYQVFQ KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR TLEAFHDTCR Q
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Med27 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MED27 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MED27 (Mediator Complex Subunit 27 (MED27))
    Autre désignation
    Med27 (MED27 Produits)
    Synonymes
    CRAP34 Protein, CRSP34 Protein, CRSP8 Protein, TRAP37 Protein, CG1245 Protein, Dmel\\CG1245 Protein, Med27 Protein, Trap37 Protein, dCRSP34 Protein, dCrsp34 Protein, dTRAP37 Protein, med27 Protein, crsp8 Protein, zgc:66302 Protein, MED27 Protein, DDBDRAFT_0216764 Protein, DDBDRAFT_0266847 Protein, DDB_0216764 Protein, DDB_0266847 Protein, 1500015J03Rik Protein, 2310042P07Rik Protein, AA682045 Protein, Crsp8 Protein, D2Ertd434e Protein, RGD1564993 Protein, DKFZp459I1912 Protein, CRSP complex subunit 8-B Protein, Xcrsp34 Protein, crap34 Protein, crsp34 Protein, crsp8-B Protein, med27-a Protein, med27-b Protein, trap37 Protein, mediator complex subunit 27 Protein, Mediator complex subunit 27 Protein, cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa Protein, hypothetical protein Protein, mediator complex subunit 27 S homeolog Protein, MED27 Protein, med27 Protein, crsp8 Protein, Med27 Protein, med27.S Protein
    Sujet
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). {ECO:0000250}.
    Poids moléculaire
    36.3 kDa Including tag.
    UniProt
    Q9DB40
    Pathways
    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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