ARFGAP1 Protein (AA 1-414) (Strep Tag)

N° du produit ABIN3129742

Détail du produit

Antigène: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-414

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ARFGAP1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLETQ DDYEPSWSLQ DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRAS GQPQSAAASG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL NNAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED SSDRPLEGHS YQNSSGDNSQ NSNIDQSFWE TFGSAEPPKA KSPSSDSWTC ADASTGRRSS DSWDVWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA APSTADEGWD NQNW
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))

Autre désignation: Arfgap1 (ARFGAP1 Produits)

Synonymes: ARF1GAP Protein, HRIHFB2281 Protein, AI115377 Protein, Arf1gap Protein, Arf GAP1 Protein, arf1gap Protein, atfb1 Protein, atfb3 Protein, jlns1 Protein, k(v)7.1 Protein, kcna8 Protein, kcna9 Protein, kv1.9 Protein, kv7.1 Protein, kvlqt1 Protein, lqt Protein, lqt1 Protein, rws Protein, sqt2 Protein, wrs Protein, ARFGAP3 Protein, ADP ribosylation factor GTPase activating protein 1 Protein, ADP-ribosylation factor GTPase activating protein 1 Protein, ADP ribosylation factor GTPase activating protein 1 S homeolog Protein, putative ADP-ribosylation factor GTPase activating protein 1 Protein, ATP-dependent RNA helicase DBP9 Protein, ARFGAP1 Protein, Arfgap1 Protein, arfgap1.S Protein, Tb11.01.6060 Protein, arfgap1 Protein, LMJF_32_1230 Protein, MGYG_02419 Protein

Sujet: ADP-ribosylation factor GTPase-activating protein 1 (ARF GAP 1) (ADP-ribosylation factor 1 GTPase-activating protein) (ARF1 GAP) (ARF1-directed GTPase-activating protein),FUNCTION: GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity). {ECO:0000250}.

Poids moléculaire: 45.3 kDa

UniProt: Q9EPJ9

Pathways: Signalisation p53, ER-Nucleus Signaling, Unfolded Protein Response