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C-Type Lectin Domain Family 6, Member A (CLEC6A) (AA 44-209) protein (His tag)

Crystallography grade CLEC6A Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, Crys, WB
N° du produit ABIN3130235
  • Antigène Voir toutes C-Type Lectin Domain Family 6, Member A (CLEC6A) Protéines
    C-Type Lectin Domain Family 6, Member A (CLEC6A)
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 44-209
    Origine
    • 3
    • 2
    • 1
    • 1
    Souris
    Source
    • 3
    • 2
    • 2
    Cellules d'insectes
    Purification/Conjugué
    His tag
    Application
    ELISA, SDS-PAGE (SDS), Crystallization (Crys), Western Blotting (WB)
    Séquence
    IMDQPSRRLY ELHTYHSSLT CFSEGTMVSE KMWGCCPNHW KSFGSSCYLI STKENFWSTS EQNCVQMGAH LVVINTEAEQ NFITQQLNES LSYFLGLSDP QGNGKWQWID DTPFSQNVRF WHPHEPNLPE ERCVSIVYWN PSKWGWNDVF CDSKHNSICE MKKIYL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Clec6a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CLEC6A Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    C-Type Lectin Domain Family 6, Member A (CLEC6A)
    Autre désignation
    Clec6a (CLEC6A Produits)
    Synonymes
    CLEC4N Protein, CLECSF10 Protein, CLEC6A Protein, C-type lectin domain containing 6A Protein, C-type lectin domain family 6, member A Protein, CLEC6A Protein, Clec6a Protein
    Sujet
    Binds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway. {ECO:0000269|PubMed:17050534, ECO:0000269|PubMed:19124755, ECO:0000269|PubMed:19703985, ECO:0000269|PubMed:20493731, ECO:0000269|PubMed:21059925}.
    Poids moléculaire
    20.4 kDa Including tag.
    UniProt
    Q9JKF4
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