GPATCH8 Protein (AA 1-1505) (Strep Tag)

N° du produit ABIN3130685

Détail du produit

Antigène: GPATCH8 (G Patch Domain Containing 8 (GPATCH8))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1505

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette GPATCH8 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MADRFSRFNE DRDFQGNHFD QYEEGHLEIE QASLDKPIES DNIGHRLLQK HGWKLGQGLG KSLQGRTDPI PIVVKYDVMG MGRMEMELDY AEDATERRRV LEVEKEDTEE LRQKYKDYVD KEKAIAKALE DLRANFYCEL CDKQYQKHQE FDNHINSYDH AHKQRLKDLK QREFARNVSS RSRKDEKKQE KALRRLHELA EQRKQAECAP GSGPMFRPTT VAVDEDGGEE DKDESSTNSG ASAVSSCGFG ADFSTDKGGS FTSVQITNTT GLSQAPGLAS QGISFGIKNN LGPPLQKLGV SFSFAKKAPV KLESIASVFK DHAEEGSSED GTKADEKSSD QGVQKVGDTD GTGNLDGKKE DEDPQDGGSL ASTLSKLKRM KREEGTGATE PEYYHYIPPA HCKVKPNFPF LLFMRASEQM EGDHSAHSKS APENRKSSSP KPQGCSKTAA SPGAERTVSE ASELQKEAAV AGPSEPGGKT ETKKGSGGGE DEQSVESRET SESPMCESNP KDISQATPAT KAGQGPKHPT GPFFPVLSKD ESTALQWPSE LLIFTKAEPS ISYSCNPLYF DFKLSRNKDA KAKGTEKPKD VAGSSKDHLQ SLDPREPNKS QEEEQDVVLS SEGRVDEPAS GAACSSLNKQ EPGGSHMSET EDTGRSHPSK KEPSGKSHRH KKKKKHKKSS KHKRKHKADT EEKSSKAESG EKSKKRKKRK RKKNKSSAAA DSERGPKSEP PGSGSPAPPR RRRRAQDDSQ RRSLPAEEGN SGKKDDGGGG SSCQDHSGRK HKGEPPTSSC QRRANTKHSS RSSHRSQPSS GDEDSDDASS HRLHQKSPSQ YSEEEEEEEE EEEEEDEDSG SEHSRSRSRS GHRHSSHRSS RRSYSSSSDA SSDQSCYSRQ HSYSDDSYSD YSDRSRRHSK RSHDSDDSDY TSSKHRSKRH KYSSSDDDYS LSCSQSRSRS RSHTRERSRS RGRSRSSSCS RSRSKRRSRS TTAHSWQRSR SYSRDRSRST RSPSQRSGSR KGSWGHESPE ERRSGRRDFI RSKIYRSQSP HYFQSGRGEG PGKKEDGRGD DSKGAGLPSQ NSNTGTGRGS ESDCSPEDKN SVTARLLLEK IQSRKVERKP NVCEEVLATP NKAGLKYKNP PQGYFGPKLP PSLGNKPVLP MIGKLPATRK SNKKCEESGL ERGEEQEHSE PEEGSPRSSD APFGHQFSEE AAGPLSDPPP EEPKSEEATA DHSVAPLGTP AHTDCYPGDP AISHNYLPDP SDGDTLESLD SGSQPGPVES SLLPIAPDLE HFPNYAPPSG EPSIESTDGT EDASLAPLES QPITFTPEEM EKYSKLQQAA QQHIQQQLLA KQVKAFPAST ALAPATPALQ PIHIQQPATA SATSITTVQH AILQHHAAAA AAAIGIHPHP HPQPLAQVHH IPQPHLTPIS LSHLTHSIIP GHPATFLASH PIHIIPASAI HPGPFTFHPV PHAALYPTLL APRPAAAAAT ALHLHPLLHP IFSGQDLQHP PSHGT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: GPATCH8 (G Patch Domain Containing 8 (GPATCH8))

Autre désignation: Gpatch8 (GPATCH8 Produits)

Synonymes: GPATC8 Protein, KIAA0553 Protein, 5430405G24Rik Protein, AU018890 Protein, ENSMUSG00000075516 Protein, Fbm1 Protein, Gpatc8 Protein, mKIAA0553 Protein, G-patch domain containing 8 Protein, G patch domain containing 8 Protein, GPATCH8 Protein, Gpatch8 Protein

Sujet: G patch domain-containing protein 8

Poids moléculaire: 165.0 kDa

UniProt: A2A6A1