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FRMPD4 Protein (AA 1-1320) (His tag)

Crystallography grade FRMPD4 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3130737
  • Antigène Voir toutes FRMPD4 Protéines
    FRMPD4 (FERM and PDZ Domain Containing 4 (FRMPD4))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1320
    Origine
    Souris
    Source
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette FRMPD4 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MDVFSFVKIP KLSSHRTKSS GWPPPSGTWG LNQVPPYGWE MMTNRDGRDY FINHMTQAIP FDDPRFDSCQ IIPPAPRKVE MRRDPVLGFG FVAGSEKPVV VRSVTPGGPS EGKLIPGDQI VMINDEAVSA APRERVIDLV RSCKESILLT VIQPYPSPKS AFISAAKKAR LKSNPVKVRF SEEVIINGQV SETVKDNSLL FMPNVLKVYL ENGQTKSFRF DCSTSIKDVI LTLQEKLSIK GIEHFSLMLE QRIEGAGTKL LLLHEQETLT QVTQRPSSHK MRCLFRISFV PKDPIDLLRR DPVAFEYLYV QSCNDVVQER FGPELKYDIA LRLAALQMYI ATVTTKQTQK ISLKYIEKEW GLETFLPSAV LQSMKEKNIK KALSHLVKAN QNLVPPGKKL SALQAKVHYL KFLSDLRLYG GRVFKATLVQ AEKRSEVTLL VGPRYGISHV INTKTNLVAL LADFSHVNRI EMFTEEESLV RVELHVLDVK PITLLMESSD AMNLACLTAG YYRLLVDSRR SIFNMANKKN AGTQDTGSEN KGKHNLLGPD WNCMPQMTTF IGEGEQEAQI TYIDSKQKTV EMTDSTLCPK EHRHLYIDNS YSSDELNQPL TQPGDAPCEA DYRSLAQRSL LTLSGPDTLK KAQESPRGAK VSFIFGDLAL DDGMSPPTIG YERMLEENPE MLEKQRNLYI SSANDMKNLD LTPDTDSIQF VANSVYANIG DVKNFEAPEG IEEPLLHDIC YAENTDDAED EDEVSCEEDL VVGEMNQPAI LDLSGSSDDI IDLTTLPPPE GDDNEDDFLL RSLNMAIAAP PPGFRDSSDE EDTQSQATSF HEDKEQGSSL QNEEIPVSLI DAVPTSAEGK CEKGLDPAVV STLEALEALS EEQQKSENSG VAILRAYSPE SSSDSGNETN SSEMTEGSEL AAAQKQSESL SRMFLATHEG YHPLAEEQTE FPTSKAPSVG LPPKSSHGLA ARPATDLPPK VVPSKQILHS DHMEMEPETM ETKSVTDYFS KLHMGSVAYS CTSKRKSKLP EGEGKSPLSG NIPGKKQQGT KIAEIEEDTK GKAGTVSSRD NPHLSTFNLE RTAFRKDSQR WYVASDGGVV EKSGMEAPAM KVFPRGPGLG NREAEGKEDG TVEGGADDAS VLGQGDRFLT DMACVASAKD LDNPEDTDSP SCDHATKLSE AEDNVARLCD YHLAKRMSSL QSEGHFSLQS SQGSSVDTGC GPGSSSSACA TPVESPLCPS MGKHMIPDAS GKGGRYISPE ERAPGHPNHG ATFEELHPQT EGMCPRMTVP ALHTAINADP LFGTLRDGCH RLPKIKETTV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Frmpd4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product FRMPD4 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    FRMPD4 (FERM and PDZ Domain Containing 4 (FRMPD4))
    Autre désignation
    Frmpd4 (FRMPD4 Produits)
    Synonymes
    RGD1566031 Protein, PDZD10 Protein, PDZK10 Protein, Gm196 Protein, PKAP1 Protein, Pdzd10 Protein, Pdzk10 Protein, Preso1 Protein, FERM and PDZ domain containing 4 Protein, Frmpd4 Protein, FRMPD4 Protein, frmpd4 Protein
    Sujet
    Positive regulator of dendritic spine morphogenesis and density. Required for the maintenance of excitatory synaptic transmission. Binds phosphatidylinositol 4,5-bisphosphate. {ECO:0000250|UniProtKB:Q14CM0}.
    Poids moléculaire
    145.9 kDa Including tag.
    UniProt
    A2AFR3
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