TTLL3 Protein (AA 1-927) (Strep Tag)

N° du produit ABIN3130890

Détail du produit

Antigène: TTLL3 (Tubulin tyrosine Ligase-Like Family, Member 3 (TTLL3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-927

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TTLL3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MQGVSSALLL SAGQLGPGAA WYRQEGSSEC SWLRRSQPSE LRTNFSSRWP WPRNSESRRS ERLQWPGPAS AKPEVASCGD SRRDYSSLPA RHLSSARESS MPGALGTVNP QPVRTLVPPT LDEPLPDALR PPDDSLLLWR GLTKGPNHMG RLRNAKIHVE RAVKQKKIFM IHGRYPVIRC LLRQRGWVEK KMVHPPGTAL PAPQKDLDSS MLGDSDATED EDEEENEMFR ESQLLDLDGF LEFDDLDGIH ALMSRMVRNE TPYLIWTTRR DVLDCRFLSK DQMINHYARA GSFTTKVGLC LNLRNLPWFD EADADSFFPR CYRLGAEDDK KAFIEDFWLT AARNVLKLVV KLEEKSQSIS IQAREEEAPE DTQPKKQEKK LVTVSSDFVD EALSACQEHL SSIAHKDIDK DPNSPLYLSP DDWSQFLQRY YQIVHEGAEL RYLEVQVQRC EDILQQLQNV VPQLDMEGDR NIWIVKPGAK SRGRGIMCMN RLDEMLKLVD CNPMLMKDGK WIVQKYIERP LLIFGTKFDL RQWFLVTDWN PLTVWFYRDS YIRFSTQPFS LKNLDNSVHL CNNSIQRHLE ASCHRHPMLP PDNMWSSQRF QAHLQEVDAP KAWSSVIVPG MKAAVIHALQ TSQDNVQCRK ASFELYGADF VFGEDFQPWL IEINASPTMA PSTAVTARLC AGVQADTLRV VIDRRLDRSC DTGAFELIYK QPAVEVPQYV GIRLLVEGST IKKPVPVGHR RTGVRSSLPH LLTQQGSGES KDSGSPTHRS ASRKNARAES LEHTEKPEPA AVASVSGKGK KAPFHFPSLH SKAWLPSPRV HRPQGRVLRL QHDQLVGSKA LSTTGKALMT LPTAKVLMSF PPHPDLKLAP SMLKPGKVGF ELCCTTWRVV LSGGIGEEGH RQRAAPRPSS APGKGLSSTE PCSKTET
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TTLL3 (Tubulin tyrosine Ligase-Like Family, Member 3 (TTLL3))

Autre désignation: Ttll3 (TTLL3 Produits)

Synonymes: HOTTL Protein, 4833441J24Rik Protein, AI450050 Protein, zgc:136840 Protein, tubulin tyrosine ligase like 3 Protein, tubulin tyrosine ligase-like family, member 3 Protein, Ttll3 Protein, TTLL3 Protein, ttll3 Protein

Sujet: Tubulin monoglycylase TTLL3 (EC 6.3.2.-) (Tubulin--tyrosine ligase-like protein 3),FUNCTION: Monoglycylase which modifies alpha- and beta-tubulin, adding a single glycine on the gamma-carboxyl groups of specific glutamate residues to generate monoglycine side chains within the C-terminal tail of tubulin (PubMed:19524510). Not involved in elongation step of the polyglycylation reaction (PubMed:19524510). Preferentially glycylates a beta-tail peptide over the alpha-tail, although shifts its preference toward alpha-tail as beta-tail glutamylation increases (By similarity). Competes with polyglutamylases for modification site on beta-tubulin substrate, thereby creating an anticorrelation between glycylation and glutamylation reactions (PubMed:33414192). Together with TTLL8, mediates microtubule glycylation of primary and motile cilia, which is essential for their stability and maintenance (PubMed:23897886, PubMed:25180231). Involved in microtubule glycylation of primary cilia in colon which controls cell proliferation of epithelial cells and plays an essential role in colon cancer development (PubMed:25180231). Together with TTLL8, glycylates sperm flagella which regulates axonemal dynein motor activity, thereby controlling flagellar beat, directional sperm swimming and male fertility (PubMed:33414192). {ECO:0000250|UniProtKB:B2GUB3, ECO:0000269|PubMed:19524510, ECO:0000269|PubMed:23897886, ECO:0000269|PubMed:25180231, ECO:0000269|PubMed:33414192}.

Poids moléculaire: 104.4 kDa

UniProt: A4Q9E5