NFX1 Protein (AA 1-1114) (His tag)
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- Antigène Voir toutes NFX1 Protéines
- NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1114
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette NFX1 protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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MAEAPPVSGT FKFNTDAAEF IPQERKTSGL NCGTQRRLDS SRIGRRNYSS SPPCHLPRHI PYEDISAVHQ HSYASGSKPK SPQGFFQSSN KSLKNHGLQN QPWQKARNEK HQNRNKKAQG LSEQTSDTSS LESVARSESG TNPREHSPSE SEKEVVIADP RGAKPKKAAQ LTYNYGRGPK AKGRLRSEWG NRMSPKSEDE NTRPVAISHT DSSDASCRKP VVDPCVCRRN EQRRYPQKRP PWEVEGARPR PGRNPPKQES QRHINAGPKT NMSPIPKDNL RERPTKSACD TGNLAVVSKS SRRVNQEKTA VRRQDPQVLS PFPRGKQNHM LKNVETHTGS LIEQLTTEKY ECMVCCELVQ VTAPVWSCQS CFHVFHLNCI KKWARSPASH ADGQSGWRCP ACQNVSAHVP NTYTCFCGKV KNPEWSRNEI PHSCGEVCRK KQPGQDCPHS CNLLCHPGPC PPCPAFTTKT CECGRTRHTV RCGQPVSVHC SNACENILNC GQHHCAELCH GGQCQPCRII LNQVCYCGST SRDVLCGTDV GKSDGFGDFS CLKICGKDLK CGSHTCSQVC HPQPCQPCPR LPHLVRYCPC GQTPLSQLLE HGSNARKTCM DPVPSCGKVC GKPLACGSSD FIHTCEKLCH EGDCGPCSRT SVISCRCSFR TKELPCTSLK SEDATFMCDK RCNKKRLCGR HKCNEICCVD KEHKCPLICG RKLRCGLHRC EEPCHRGNCQ TCWQASFDEL TCHCGASVIY PPVPCGTRPP ECTQTCARIH ECDHPVYHSC HSEEKCPPCT FLTQKWCMGK HELRSNIPCH LVDISCGLPC SAMLPCGMHK CQRLCHKGEC LVDEACKQPC TTPRGDCGHP CMAPCHPSLP CPVTACKAKV ELQCECGRRK EMVICSEASG TYQRIVAISM ASKITDMQLG DSVEISKLIT KKEVQQARLQ CDEECAALER RKRLAEAFDI TDDSDPFNVR SSASKFSDSL KDDARKDLKF VSDVEKEMET LVEAVNKGKN NKKSHCFPPM NRDHRRIIHD LAQVYGLESI SYDSEPKRNV VVTAVRGKSV CPPTTLTSVI ERETQTRPPP PIPHHRHQAD KAPGSSTLQK IVKEAVIDYF DVQD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Nfx1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product NFX1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- NFX1 (Nuclear Transcription Factor, X-Box Binding 1 (NFX1))
- Autre désignation
- Nfx1 (NFX1 Produits)
- Synonymes
- NFX1 Protein, NFX2 Protein, 1300017N15Rik Protein, 3000003M19Rik Protein, NFX.1 Protein, TEG-42 Protein, Tex42 Protein, nuclear transcription factor, X-box binding 1 Protein, nuclear transcription factor, x-box binding 1 Protein, NFX1 Protein, CpipJ_CPIJ011986 Protein, nfx1 Protein, Nfx1 Protein
- Sujet
- Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Together with PABPC1 or PABPC4, acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination. {ECO:0000269|PubMed:12047746}.
- Poids moléculaire
- 124.8 kDa Including tag.
- UniProt
- B1AY10
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