TRAPPC11 Protein (AA 1-1133) (His tag)
-
- Antigène Voir toutes TRAPPC11 Protéines
- TRAPPC11 (Trafficking Protein Particle Complex 11 (TRAPPC11))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1133
- Origine
- Souris
-
Source
- Cellules d'insectes
- Purification/Conjugué
- Cette TRAPPC11 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
-
MSPTQWDFPV ELCCRPMAFV TLTGLDVVYN AVHRAVWDAF CANRRADRVP ISFKVLPGDH EYPKCRSKRT SYEWYIPKGV LKTGWMNKHL NLVPALVVVF YELDWDEPQW KEKQSECATR VEIVRQSLQG RNTKVAVVLI QKKTPLPPGE DVIASERAAA LCNVCELSGK SLFVLPHTDH LVGYIIRLEN AFYEHAQTYY YTEIRRVKSH KEFLNKTTHQ LLFVRHQFKI AFFSELKQDT QNALKNYRIA YNLVHELRAH ETNILEIKTM AGFINYKICR LCFQHNTPLD AIAQFRKHID LCKKKIGSAE LAFEHAAWMA KQFQAFGDLF DEAIKLGLTA IQTQNPGFYY QQAAYYAQER KQHAKALCNH DAAVMYPNPD PLETQSGVLD FYGQRPWRQG ILSFDLSDPE KEKAGILAIQ LKERSVVHSE IIIALLSNAV AQFKKYKCPR MKSHLMVQMG EEYYYAKDYT KALKLLDYVM CDYRSEAWWT LLTSILTTAL KCSYLMAQLK DYITYSLELL GRASTLKDEQ KSRIEKNLMN VLMNESPDPE PDCDVLAVKT AQKLWADRVS LAGSNVFQIG VQDFVPFVQC KAKFHAPSFH VDVPVEFDVF LKADCPHPIR FSKLCVSFNN QVYNQFCVLE EASKASEVLE NLTQGKMCLV PGKTRKLSFK FVAKTEDVGK KIEITSVDLF LGSESGRCVV LSWQGGGGDA ASSQEALQAA RSFKRRPKLA EDEIHWDSVI IQASTMIISR VPNISVHLRH EPPALMNEMY CLVVTVQSHE KSPIRDVKLT AGLKPGQDAN LTQKTHVTLH GAELCDESYP ALLTDIPVGD LHPGEQLEKT VYVRCGTVGS RMFLVYVSYL INTTVEGKEI ICKCHKDDTV TIETVFPFDV AVRFVSTKFE HLERVYADIP FLLMTDVLSA SPWALTIVSS ELQLAPSMTA MDHLESQIDK VVLQTGESAS ECFCLRCPSA GNIEGGVATG HYIISWKRAS VVESIPAVST VITLPHVIAE NIPLHVNADL PSFGRVRESL PVRYHLQNKT DLVQDVEISV EPSDAFMFSG LKQIRLRILP GTKQEMLYNF YPLMAGYQQL PSLNINLLRF PNFTNQLLRR FIPTSIFVKP QGRLLEDTSI AAA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Trappc11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product TRAPPC11 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- TRAPPC11 (Trafficking Protein Particle Complex 11 (TRAPPC11))
- Autre désignation
- Trappc11 (TRAPPC11 Produits)
- Synonymes
- TRAPPC11 Protein, C4orf41 Protein, FOIGR Protein, GRY Protein, LGMD2S Protein, C27H4orf41 Protein, AW558465 Protein, D030016E14Rik Protein, R75422 Protein, fb78g05 Protein, fgr Protein, flj12716l Protein, foigr Protein, wu:fb78g05 Protein, zgc:136723 Protein, zgc:63684 Protein, trafficking protein particle complex 11 Protein, TRAPPC11 Protein, Trappc11 Protein, trappc11 Protein
- Sujet
- Involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage. {ECO:0000250}.
- Poids moléculaire
- 129.4 kDa Including tag.
- UniProt
- B2RXC1
-