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CCDC78 Protein (AA 1-437) (His tag)

Crystallography grade CCDC78 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
N° du produit ABIN3131109
  • Antigène Voir toutes CCDC78 Protéines
    CCDC78 (Coiled-Coil Domain Containing 78 (CCDC78))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-437
    Origine
    Souris
    Source
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette CCDC78 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Séquence
    MDQRPELLSS MEYVASPDPK PGVPLRVAEN VAPGAEDWLP SASGHLAWAT SLETEHQTHL ELSEEQRLQI SKELVDLQIA THHLREQHEA EVFELRREIL RLESRVLELE LHGNGACQGH KVQPMANLGQ HQVPPLEPPG GQQKLQEELK WLLEHHRARQ QALETQVGVL SQQLQGAREE ARTTGQQLAS QAMVLASCKG QLRQAEAENT QLQLQLKKMN EEYAVRLQHY ARETVENASS TNQAALQAFL ESTLQDIRAA HRTREQQLAQ AARTYRKRLA DLNQRQELLL TTCRATFATA INLEPLPMHW ATELSHPREN EYGRHRTLLL YPEKGSGETS KENKSQPLAL DTASWAQIQQ RLQDFSQDTQ AELERERAQL MVRATMAEQQ LSELQEYVDQ HLGRYKQEIL KLRKLVNIGD PQGVEAVSSP GSGGARL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ccdc78 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CCDC78 (Coiled-Coil Domain Containing 78 (CCDC78))
    Autre désignation
    Ccdc78 (CCDC78 Produits)
    Synonymes
    MGC86539 Protein, C16orf25 Protein, CNM4 Protein, JFP10 Protein, Gm938 Protein, Gm950 Protein, coiled-coil domain containing 78 S homeolog Protein, coiled-coil domain containing 78 Protein, ccdc78.S Protein, CCDC78 Protein, ccdc78 Protein, Ccdc78 Protein
    Sujet
    Component of the deuterosome, a structure that promotes de novo centriole amplification in multiciliated cells that can generate more than 100 centrioles. Deuterosome-mediated centriole amplification occurs in terminally differentiated multiciliated cells (G1/0) and not in S phase. Essential for centriole amplification and is required for CEP152 localization to the deuterosome (By similarity). {ECO:0000250}.
    Poids moléculaire
    50.4 kDa Including tag.
    UniProt
    D3Z5T1
    Pathways
    Skeletal Muscle Fiber Development
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