KIAA0586 Protein (KIAA0586) (AA 1-1520) (Strep Tag)

N° du produit ABIN3131129

Détail du produit

Antigène: KIAA0586

Type de proteíne: Recombinant

Attributs du protein: AA 1-1520

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette KIAA0586 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MKNVEFSLER GQRLKMPARK LREIVSPNQG NKLAVVEDEL PRVPPALAAN KRLAVETRTS SNGTLCGSLD LTSARLYHQP LLESPPASKK SDFSKDAVVR QLPLNKTEEN NAPKANDIFI SQYTMGQKDA LRTVLKQKAQ SMPVFKAVKV HLFEDTSTEK NTVAQETETP PNRIDSATTV AAATAAAIAT AAPLIKVQSD LEAKVNCVGE LLTKLQETDK QLQRVTEHQA SVQSKQEKVH CHDHDKQMNA FMEQHIRHLE KLQQQQIDIQ THFIDAALKA SSLQLGMSTS RAVGKYSGKL GSPSVGSSVF SHNTFVSKRV PLSEDTDFDG QKSPLETPAP RRFAPVPVSR DGKITKRESP TEEKENMEMN SPKGNVRLLE QVLNSNECLT RKTESSDITS LTQPKMGWNL EKRDSTETLH SQIFPSSEER GTAQVPVPKY NDVVHDLGQK KQASDMLQIK QSPVTLRLSD HPHNPALLQT TNTRSVLKDA AKILRGVQNN KKVLEENLEA IVRAKDGAAM YSFINALATN REMSEKIRIR KQVDEWIKII SAEIQDELMR KDYEQKRFDP KNQRNKKALT MSRDIKANNQ EKTVNRSVIP RSHYQKQTQE QFTSPPVRNL PASGPQKERS GLLKSATTLQ DEDYMLQIYG KPVYQGHRST LKKGPYLRFS SPSPKAKPQR PRVIELVKGT KVKSAKTQTD FHAASRMKMD SKIQHPITAL PHADQQYMVS PSREMPTVSG TLEGHLIPMA ILLGQTQSNS DSMPPAGVTV NKPRPVTVTT SIPPASRKGN AGVKKPNVAI VEMKSEKKDP PQLSVQILPS VDIDSVSYSS TDGASSPPSP KEASLPPLHT WIQTPDFMKV DEEEVPLPGT NFDEVIDVIQ EEEKRDEIPE CSAPMLEFNR SVKVVPTKYN GPSFPPVVSA YHPTTDILDK VIERKETLEN SLIQWVEQEI MSRIISGLFP LQQQARLDAS VSVSEASEPS ASDIVAGTSS GALQRMVDAR VPVNSDMVSH FVNEALTETI AVMLADREAE RQRAAATSVP GDLSGTETNL LARVCAPVAT PQPTPPCSPS PVREHVRVKT PDSSPCESDP DAASSIKEIR VEKGSDMPAV MLVSTPTRTP VATPPPAAAL TPTLSETSID KLKLSSPELP KPWDSGDLPL DEENPNSLQE LPHPRAVVMS VANEEPESVD FSAQPAPPEP APSAPLPEGT KAPSLQRVPS SGSSTLENTL STVTETETLD RHISEGEILF SCGQNLATKR PGDLFLMNIN DSLSSTLQDA LEMEDDPPSE GQVIRRPHKK RHEDAIVALL TKQQRELLVS QQEEDLDNSV GELSEGQRLV LKAAEDISAG PSGQMLPPTS PAEPSYQHAD PRLVLQQSDM ASGNICEDLC ASHGPMSLRE LELQPDSNLI LPITHTTTAV SDGNLPEAAE DFSQYQQKQD SDIKQVEHKP IQRHLTSVRN KPDSTLSQHQ GGPADLLLIA HVSPARMSVT LPSANLEDCS QSLSTSSMHG GTESSGTDTF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: KIAA0586

Autre désignation: Talpid3 (KIAA0586 Produits)

Synonymes: Talpid3 Protein, KIAA0586 Protein, KIAA0586 Protein

Sujet: Protein TALPID3,FUNCTION: Required for ciliogenesis and sonic hedgehog/SHH signaling (PubMed:21750036). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1. May play a role in early ciliogenesis in the disappearance of centriolar satellites that preceeds ciliary vesicle formation (By similarity). Involved in regulation of cell intracellular organization (PubMed:26386247). Involved in regulation of cell polarity (By similarity). Required for asymmetrical localization of CEP120 to daughter centrioles (PubMed:25251415). {ECO:0000250|UniProtKB:Q1G7G9, ECO:0000269|PubMed:21750036, ECO:0000269|PubMed:25251415, ECO:0000269|PubMed:26386247}.

Poids moléculaire: 166.7 kDa

UniProt: E9PV87