CLTA Protein (AA 1-235) (Strep Tag)

N° du produit ABIN3131254

Détail du produit

Antigène: CLTA (Clathrin, Light Chain A (CLTA))

Type de proteíne: Recombinant

Attributs du protein: AA 1-235

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CLTA protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP GRATRRAAGG PDAVDGVMNG EYYQESNGPT DSYAAISEVD RLQSEPESIR KWREEQTERL EALDANSRKQ EAEWKEKAIK ELEEWYARQD EQLQKTKANN RVADEAFYKQ PFADLIGYVA AEEAFVNDID ESSPGTEWER VARLCDFNPK SSKQAKDVSR MRSVLISLKQ APLVH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CLTA (Clathrin, Light Chain A (CLTA))

Autre désignation: Clta (CLTA Produits)

Synonymes: lca Protein, zgc:73358 Protein, wu:fb61d08 Protein, wu:fj48d06 Protein, CLTA Protein, DDBDRAFT_0169251 Protein, DDBDRAFT_0191305 Protein, DDB_0169251 Protein, DDB_0191305 Protein, clc Protein, LCA Protein, LCA1 Protein, LCA2 Protein, LCA3 Protein, CLTA2 Protein, AV026556 Protein, Clathrin light chain Protein, clathrin light chain A Protein, clathrin, light chain A Protein, clathrin, light chain (Lca) Protein, clathrin light chain Protein, clathrin, light polypeptide (Lca) Protein, clic-1 Protein, clta Protein, CLTA Protein, CNI00580 Protein, LOC5567495 Protein, CLC1 Protein, CpipJ_CPIJ018056 Protein, PTRG_01988 Protein, PMAA_096950 Protein, SJAG_04517 Protein, TSTA_044110 Protein, PAS_chr1-4_0158 Protein, clc Protein, Clta Protein

Sujet: Clathrin light chain A (Lca),FUNCTION: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge (By similarity). {ECO:0000250|UniProtKB:P09496}.

Poids moléculaire: 25.6 kDa

UniProt: O08585

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation