Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Antizyme Inhibitor 1 Protein (AZIN1) (AA 1-448) (His tag)

Crystallography grade AZIN1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3131571
  • Antigène Voir toutes Antizyme Inhibitor 1 (AZIN1) Protéines
    Antizyme Inhibitor 1 (AZIN1)
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-448
    Origine
    • 6
    • 2
    • 1
    Souris
    Source
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette Antizyme Inhibitor 1 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    MKGFIDDANY SVGLLDEGTN LGNVIDNYVY EHTLTGKNAF FVGDLGKIVK KHSQWQTVVA QIKPFYTVKC NSTPAVLEIL AALGTGFACS SKNEMALVQE LGVSPENIIF TSPCKQVSQI KYAAKVGVNI MTCDNEIELK KIARNHPNAK VLLHIATEDN IGGEDGNMKF GTTLKNCRHL LECAKELDVQ IIGVKFHVSS ACKEYQVYVH ALSDARCVFD MAGEFGFTMN MLDIGGGFTG TEIQLEEVNH VISPLLDIYF PEGSGIQIIS EPGSYYVSSA FTLAVNIIAK KVVENDKFSS GVEKNGSDEP AFVYYMNDGV YGSFASKLSE DLNTIPEVHK KYKEDEPLFT SSLWGPSCDE LDQIVESCLL PELNVGDWLI FDNMGADSFH EPSAFNDFQR PAIYFMMSFS DWYEMQDAGI TSDAMMKNFF FAPSCIQLSQ EDSFSTEA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Azin1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product AZIN1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Antizyme Inhibitor 1 (AZIN1)
    Autre désignation
    Azin1 (AZIN1 Produits)
    Synonymes
    OAZI Protein, OAZIN Protein, ODC1L Protein, 1700085L02Rik Protein, AI414949 Protein, Oazi Protein, Oazin Protein, AZIN1 Protein, azin1 Protein, oazin Protein, wu:fj32d12 Protein, MGC81852 Protein, wu:fc93f10 Protein, zgc:110131 Protein, antizyme inhibitor 1 Protein, antizyme inhibitor 1b Protein, antizyme inhibitor 1 L homeolog Protein, antizyme inhibitor 1a Protein, AZIN1 Protein, Azin1 Protein, azin1b Protein, azin1.L Protein, azin1 Protein, azin1a Protein
    Sujet
    Antizyme inhibitor protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake by counteracting the negative effect of antizyme OAZ1, OAZ2 and OAZ3 on ODC1 activity (PubMed:16916800, PubMed:18062773, PubMed:18508777). Inhibits antizyme-dependent ODC degradation by binding to antizymes (PubMed:18062773). Releases ODC1 from its inactive complex with antizymes, leading to formation of the catalytically active ODC1. {ECO:0000269|PubMed:10698696, ECO:0000269|PubMed:16916800, ECO:0000269|PubMed:18062773, ECO:0000269|PubMed:18369191, ECO:0000269|PubMed:18508777}.
    Poids moléculaire
    50.5 kDa Including tag.
    UniProt
    O35484
Vous êtes ici:
Support technique