COPS5 Protein (AA 2-334) (His tag)

N° du produit ABIN3131689

Détail du produit

Antigène: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Type de proteíne: Recombinant

Attributs du protein: AA 2-334

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette COPS5 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AASGSGMAQK TWELANNMQE AQSIDEIYKY DKKQQQEILA AKPWTKDHHY FKYCKISALA LLKMVMHARS GGNLEVMGLM LGKVDGETMI IMDSFALPVE GTETRVNAQA AAYEYMAAYI ENAKQVGRLE NAIGWYHSHP GYGCWLSGID VSTQMLNQQF QEPFVAVVID PTRTISAGKV NLGAFRTYPK GYKPPDEGPS EYQTIPLNKI EDFGVHCKQY YALEVSYFKS SLDRKLLELL WNKYWVNTLS SSSLLTNADY TTGQVFDLSE KLEQSEAQLG RGSFMLGLET HDRKSEDKLA KATRDSCKTT IEAIHGLMSQ VIKDKLFNQI NVA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cops5 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: COPS5 (COP9 Constitutive Photomorphogenic Homolog Subunit 5 (Arabidopsis) (COPS5))

Autre désignation: Cops5 (COPS5 Produits)

Synonymes: BcDNA.LD14392 Protein, BcDNA:LD14392 Protein, CG14884 Protein, CH5 Protein, CSN-5 Protein, Csn5 Protein, DCH5 Protein, Dch5 Protein, Dmel\\CG14884 Protein, JAB1 Protein, JAB1/CSN5 Protein, Jab1 Protein, JadBp Protein, csn5 Protein, dch5 Protein, jab1/csn5 Protein, l(3)89Cf Protein, l(3)L4032 Protein, l(3)M203 Protein, quo Protein, COPS5 Protein, MGC76063 Protein, AI303502 Protein, CSN5 Protein, Mov34 Protein, Sgn5 Protein, zgc:73130 Protein, MOV-34 Protein, SGN5 Protein, COP9 signalosome subunit 5 Protein, COP9 signalosome complex subunit 5 Protein, COP9 signalosome subunit 5 S homeolog Protein, CSN5 Protein, COPS5 Protein, cops5 Protein, CpipJ_CPIJ012314 Protein, Bm1_46585 Protein, VDBG_06932 Protein, LOAG_01443 Protein, csn5 Protein, Cops5 Protein, cops5.S Protein, csn-5 Protein

Sujet: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. Promotes the proteasomal degradation of BRSK2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex.

Poids moléculaire: 38.4 kDa Including tag.

UniProt: O35864

Pathways: Cycle Cellulaire