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Periaxin Protein (PRX) (AA 1-1391) (His tag)

Crystallography grade PRX Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
N° du produit ABIN3131869
  • Antigène Voir toutes Periaxin (PRX) Protéines
    Periaxin (PRX)
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1391
    Origine
    Souris
    Source
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette Periaxin protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Séquence
    MEARSRSAEE LRRAELVEII VETEAQTGVS GFNVAGGGKE GIFVRELRED SPAAKSLSLQ EGDQLLSARV FFENFKYEDA LRLLQCAEPY KVSFCLKRTV PTGDLALRPG TVSGYEMKGP RAKVAKLNIQ SLAPVKKKKM VTGALGTPAD LAPVDVEFSF PKFSRLRRGL KAEAVKGPVP AAPARRRLQL PRLRVREVAE EAQVARMAAA APPPRKAKAE AEAATGAGFT APQIELVGPR LPSAEVGVPQ VSVPKGTPST EAASGFALHL PTLGLGAPAA PAVEPPATGI QVPQVELPTL PSLPTLPTLP CLDTQEGAAV VKVPTLDVAA PSMGVDLALP GAEVEAQGEV PEVALKMPRL SFPRFGIRGK EATEAKVVKG SPEAKAKGPR LRMPTFGLSL LEPRPSGPEA VAESKLKLPT LKMPSFGIGV AGPEVKAPTG PEVKLPKVPE VKLPKVPEAA IPDVQLPEVQ LPKMSDMKLP KIPEMVVPDV RLPEVQLPKV PEMKVPEMKL PKWPEMAVPD VHLPDVQLPK VPEMKLPKVP EMAVPDVHLP DVQLPKVPEM KLPEMKLPKV PEMAVPDVRL PEVQLPKVSE VKLPKMPEMA VPDVHLPELQ LPKMSEVKLP KMPEMAVPDV RLPEVQLPKV SEMKLPKMPE MTMPDIRLPE VQLPKVPDIK LPEMKLPEIK LPKVPDMAVP DVPLPELQLP KVSDIRLPEM QVSQVPEVQL PKMPEMKLSK VPEVQRKSAG AEQAKGTEFS FKLPKMTMPK LGKVGKPGEA SIEVPDKLMT LPCLQPEVGT EASHVGVPSL SLPSVELDLP GALGLEGQVQ EAVPGKVEKP EGPRVAVGVG EVGFRVPSVE IVTPQLPTVE VEKEQLEMVE MKVKPSSKFS LPKFGLSGPK AVKGEVEGPG RATKLKVSKF TISLPKARAG TEAEAKGAGE AGLLPALDLS IPQLSLDAQL PSGKVEVADS KPKSSRFALP KFGVKGRDSE ADVLVAGEAE LEGKGWGWDG KVKMPKLKMP SFGLSRGKEA ETQDGRVSPG EKLEAIAGQL KIPAVELVTP GAQETEKVTS GVKPSGLQVS TTGQVVAEGQ ESVQRVSTLG ISLPQVELAS FGEAGPEIVA PSAEGTAGSR VQVPQVMLEL PGTQVAGGDL LVGEGIFKMP TVTVPQLELD VGLGHEAQAG EAAKSEGGIK LKLPTLGTGS RGEGVEPQGP EAQRTFHLSL PDVELTSPVS SHAEYQVVEG DGDGGHKLKV RLPLFGLAKA KEGIEVGEKV KSPKLRLPRV GFSQSESVSG EGSPSPEEEE EGSGEGASSR RGRVRVRLPR VGLASPSKVS KGQEGDATSK SPVGEKSPKF RFPRVSLSPK ARSGSRDREE GGFRVRLPSV GFSETAVPGS TRIEGTQAAA I
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Prx Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product PRX Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Periaxin (PRX)
    Autre désignation
    Prx (PRX Produits)
    Synonymes
    xprx Protein, MGC89167 Protein, CMT4F Protein, L-Periaxin Protein, periaxin Protein, prx Protein, PRX Protein, Prx Protein
    Sujet
    Scaffolding protein that functions as part of a dystroglycan complex in Schwann cells, and as part of EZR and AHNAK-containing complexes in eye lens fiber cells (PubMed:11430802, PubMed:21745462, PubMed:22764250). Required for the maintenance of the peripheral myelin sheath that is essential for normal transmission of nerve impulses and normal perception of sensory stimuli (PubMed:10839370). Required for normal transport of MBP mRNA from the perinuclear to the paranodal regions (PubMed:15356632). Required for normal remyelination after nerve injury (PubMed:10839370). Required for normal elongation of Schwann cells and normal length of the internodes between the nodes of Ranvier. The demyelinated nodes of Ranvier permit saltatory transmission of nerve impulses, shorter internodes cause slower transmission of nerve impulses (PubMed:15356632, PubMed:23022068). Required for the formation of appositions between the abaxonal surface of the myelin sheath and the Schwann cell plasma membrane, the Schwann cell cytoplasm is restricted to regions between these appositions (PubMed:15356632, PubMed:23022068). Required for the formation of Cajal bands and of Schmidt-Lanterman incisures that correspond to short, cytoplasm-filled regions on myelinated nerves (PubMed:23022068, PubMed:22764250). Recruits DRP2 to the Schwann cell plasma membrane (PubMed:11430802, PubMed:23022068, PubMed:22764250). Required for normal protein composition of the eye lens fiber cell plasma membrane and normal eye lens fiber cell morphology (PubMed:21745462). {ECO:0000269|PubMed:10839370, ECO:0000269|PubMed:11430802, ECO:0000269|PubMed:15356632, ECO:0000269|PubMed:22764250, ECO:0000269|PubMed:23022068}.
    Poids moléculaire
    148.6 kDa Including tag.
    UniProt
    O55103
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