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Keratin 10 Protein (KRT10) (AA 1-570) (His tag)

Crystallography grade KRT10 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3132433
  • Antigène Voir toutes Keratin 10 (KRT10) Protéines
    Keratin 10 (KRT10)
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-570
    Origine
    • 4
    • 3
    • 1
    Souris
    Source
    • 6
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette Keratin 10 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    MSVLYSSSSK QFSSSRSGGG GGGGSVRVSS TRGSLGGGYS SGGFSGGSFS RGSSGGGCFG GSSGGYGGFG GGGSFGGGYG GSSFGGGYGG SSFGGGYGGS SFGGAGFGGG GSFGGGSFGG GSYGGGFGGG GFGGDGGSLL SGNGRVTMQN LNDRLASYMD KVRALEESNY ELEGKIKEWY EKHGNSSQRE PRDYSKYYKT IEDLKGQILT LTTDNANVLL QIDNARLAAD DFRLKYENEV TLRQSVEADI NGLRRVLDEL TLSKSDLEMQ IESLNEELAY LKKNHEEEMR DLQNVSTGDV NVEMNAAPGV DLTQLLNNMR NQYEQLAEKN RKDAEEWFNQ KSKELTTEID SNIEQMSSHK SEITELRRTV QGLEIELQSQ LALKQSLEAS LAETEGRYCV QLSQIQSQIS ALEEQLQQIR AETECQNAEY QQLLDIKTRL ENEIQTYRSL LEGEGSSSGG GGGRRGGSGG GSYGGSSGGG SYGGSSGGGG SYGGSSGGGG SYGGGSSGGG SHGGSSGGGY GGGSSSGGAG GHGGSSGGGY GGGSSSGGQG GSGGFKSSGG GDQSSKGPRY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Krt10 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product KRT10 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Keratin 10 (KRT10)
    Autre désignation
    Krt10 (KRT10 Produits)
    Synonymes
    BCIE Protein, BIE Protein, CK10 Protein, EHK Protein, K10 Protein, KPP Protein, D130054E02Rik Protein, K1C1 Protein, Krt-1.10 Protein, Krt1-10 Protein, Ka10 Protein, Ker10 Protein, KRT10 Protein, KRT25 Protein, KRT27 Protein, KRT28 Protein, keratin 10 Protein, keratin 10, type I S homeolog Protein, keratin, type I cytoskeletal 25 Protein, KRT10 Protein, Krt10 Protein, krt10.S Protein, LOC101085587 Protein
    Poids moléculaire
    58.7 kDa Including tag.
    UniProt
    P02535
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