LMYC Protein (AA 1-368) (Strep Tag)

N° du produit ABIN3132790

Détail du produit

Antigène: LMYC (MYCL) (Protein L-Myc (MYCL))

Type de proteíne: Recombinant

Attributs du protein: AA 1-368

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette LMYC protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MDFDSYQHYF YDYDCGEDFY RSTAPSEDIW KKFELVPSPP TSPPWGSGPG AVDPASGINP GEPWPGGGAG DEAESRGHSK AWGRNYASII RRDCMWSGFS ARERLERVVS DRLAPGAPRG NPPKAPATPD GTPSLEASNP APATQCQLGE PKTQACSGSE SPSDSEGEEI DVVTVEKRRS LDIRKPVTIT VRADPLDPCM KHFHISIHQQ QHNYAARFPP ESCSQEGDPE PGPQEEAPEI EAPKEKEEEE EEEEEEEIVS PPPVGSEAPQ SCHPKPVSSD TEDVTKRKNH NFLERKRRND LRSRFLALRD QVPTLASCSK APKVVILSKA LEYLQALVGA EKKMATEKRQ LRCRQQQLQK RIAYLSGY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: LMYC (MYCL) (Protein L-Myc (MYCL))

Autre désignation: Mycl (MYCL Produits)

Synonymes: MRTL Protein, MYCC Protein, bHLHe39 Protein, c-Myc Protein, MYCL1 Protein, Lmyc1 Protein, Mycl1 Protein, LMYC Protein, bHLHe38 Protein, CMYC Protein, c-myc Protein, ARC Protein, B430311C09Rik Protein, MYC Protein, NOP Protein, Nop30 Protein, RNCMYC Protein, mMyc Protein, C-MYC Protein, AU016757 Protein, Myc2 Protein, Niard Protein, Nird Protein, AW536278 Protein, L-myc Protein, Lmyc-1 Protein, l-myc Protein, lmyc Protein, lmyc-b Protein, lmyc2 Protein, mycl1-b Protein, xL-Myc2 Protein, fi04c04 Protein, mycl1b Protein, si:ch211-194e15.2 Protein, wu:fa07c04 Protein, wu:fb19f03 Protein, wu:fi04c04 Protein, zgc:152988 Protein, lmyc1 Protein, mycl1 Protein, mycl1a Protein, zgc:85967 Protein, lmyc-A Protein, mycl Protein, mycl1-a Protein, xL-Myc1 Protein, xL-myc1 Protein, MYC proto-oncogene, bHLH transcription factor Protein, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived homolog Protein, MYCL proto-oncogene, bHLH transcription factor Protein, nucleolar protein 3 (apoptosis repressor with CARD domain) Protein, myelocytomatosis oncogene Protein, v-myc avian myelocytomatosis viral oncogene lung carcinoma derived Protein, MYCL proto-oncogene, bHLH transcription factor S homeolog Protein, MYCL proto-oncogene, bHLH transcription factor b Protein, MYCL proto-oncogene, bHLH transcription factor a Protein, MYCL proto-oncogene, bHLH transcription factor L homeolog Protein, MYC Protein, MYCL Protein, Mycl Protein, Nol3 Protein, Myc Protein, mycl.S Protein, myclb Protein, mycla Protein, mycl.L Protein

Sujet: Protein L-Myc

Poids moléculaire: 40.8 kDa

UniProt: P10166

Pathways: L'effet Warburg