Retinoblastoma 1 Protein (RB1) (AA 2-921) (His tag)

N° du produit ABIN3132938

Détail du produit

Antigène: Retinoblastoma 1 (RB1)

Type de proteíne: Recombinant

Attributs du protein: AA 2-921

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette Retinoblastoma 1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: PPKAPRRAAA AEPPPPPPPP PREDDPAQDS GPEELPLARL EFEEIEEPEF IALCQKLKVP DHVRERAWLT WEKVSSVDGI LEGYIQKKKE LWGICIFIAA VDLDEMPFTF TELQKSIETS VYKFFDLLKE IDTSTKVDNA MSRLLKKYNV LCALYSKLER TCELIYLTQP SSALSTEINS MLVLKISWIT FLLAKGEVLQ MEDDLVISFQ LMLCVVDYFI KFSPPALLRE PYKTAAIPIN GSPRTPRRGQ NRSARIAKQL ENDTRIIEVL CKEHECNIDE VKNVYFKNFI PFINSLGIVS SNGLPEVESL SKRYEEVYLK NKDLDARLFL DHDKTLQTDP IDSFETERTP RKNNPDEEAN VVTPHTPVRT VMNTIQQLMV ILNSASDQPS ENLISYFNNC TVNPKENILK RVKDVGHIFK EKFANAVGQG CVDIGVQRYK LGVRLYYRVM ESMLKSEEER LSIQNFSKLL NDNIFHMSLL ACALEVVMAT YSRSTLQHLD SGTDLSFPWI LNVLNLKAFD FYKVIESFIK VEANLTREMI KHLERCEHRI MESLAWLSDS PLFDLIKQSK DGEGPDNLEP ACPLSLPLQG NHTAADMYLS PLRSPKKRTS TTRVNSAANT ETQAASAFHT QKPLKSTSLA LFYKKVYRLA YLRLNTLCAR LLSDHPELEH IIWTLFQHTL QNEYELMRDR HLDQIMMCSM YGICKVKNID LKFKIIVTAY KDLPHAAQET FKRVLIREEE FDSIIVFYNS VFMQRLKTNI LQYASTRPPT LSPIPHIPRS PYKFSSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGGNP PKPLKKLRFD IEGADEADGS KHLPAESKFQ QKLAEMTSTR TRMQKQRMNE SKDVSNKEEK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Rb1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Retinoblastoma 1 (RB1)

Autre désignation: Rb1 (RB1 Produits)

Synonymes: OSRC Protein, RB Protein, p105-Rb Protein, pRb Protein, pp110 Protein, Rb Protein, Rb-1 Protein, P105-RB Protein, PP110 Protein, RB11 Protein, zgc:154147 Protein, RB transcriptional corepressor 1 Protein, RB transcriptional corepressor like 1 Protein, retinoblastoma 1 Protein, RB1 Protein, RBL1 Protein, Rb1 Protein, rb1 Protein

Sujet: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {ECO:0000250, ECO:0000269|PubMed:15750587, ECO:0000269|PubMed:16612004}.

Poids moléculaire: 106.2 kDa Including tag.

UniProt: P13405

Pathways: Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy