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CYR61 Protein (AA 25-379) (His tag)

Crystallography grade CYR61 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3133186
  • Antigène Voir toutes CYR61 Protéines
    CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 25-379
    Origine
    • 9
    • 3
    • 1
    • 1
    • 1
    Souris
    Source
    • 5
    • 4
    • 3
    • 2
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CYR61 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    TCPAACHCPL EAPKCAPGVG LVRDGCGCCK VCAKQLNEDC SKTQPCDHTK GLECNFGASS TALKGICRAQ SEGRPCEYNS RIYQNGESFQ PNCKHQCTCI DGAVGCIPLC PQELSLPNLG CPNPRLVKVS GQCCEEWVCD EDSIKDSLDD QDDLLGLDAS EVELTRNNEL IAIGKGSSLK RLPVFGTEPR VLFNPLHAHG QKCIVQTTSW SQCSKSCGTG ISTRVTNDNP ECRLVKETRI CEVRPCGQPV YSSLKKGKKC SKTKKSPEPV RFTYAGCSSV KKYRPKYCGS CVDGRCCTPL QTRTVKMRFR CEDGEMFSKN VMMIQSCKCN YNCPHPNEAS FRLYSLFNDI HKFRD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cyr61 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CYR61 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
    Autre désignation
    Cyr61 (CYR61 Produits)
    Synonymes
    CCN1 Protein, GIG1 Protein, IGFBP10 Protein, ccn1 Protein, gig1 Protein, Xcyr61 Protein, igfbp10 Protein, MGC69281 Protein, MGC69539 Protein, cyr61-l2 Protein, zgc:158158 Protein, CYR61 Protein, AI325051 Protein, Igfbp10 Protein, cysteine rich angiogenic inducer 61 Protein, cysteine-rich, angiogenic inducer, 61 Protein, cysteine-rich, angiogenic inducer, 61 like 2 Protein, cysteine rich protein 61 Protein, cysteine rich angiogenic inducer 61 S homeolog Protein, CYR61 Protein, Cyr61 Protein, cyr61 Protein, cyr61l2 Protein, cyr61.S Protein
    Sujet
    Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CYR61-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity). {ECO:0000250}.
    Poids moléculaire
    40.1 kDa Including tag.
    UniProt
    P18406
    Pathways
    Positive Regulation of Endopeptidase Activity, Growth Factor Binding
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