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ABCB4 Protein (AA 992-1276) (His tag)

Crystallography grade ABCB4 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3133295
  • Antigène Voir toutes ABCB4 Protéines
    ABCB4 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4 (ABCB4))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 992-1276
    Origine
    • 4
    • 4
    • 1
    Souris
    Source
    • 6
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette ABCB4 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    PDYAKAKLSA AYLFSLFERQ PLIDSYSGEG LWPDKFEGSV TFNEVVFNYP TRANVPVLQG LSLEVKKGQT LALVGSSGCG KSTVVQLLER FYDPMAGSVL LDGQEAKKLN VQWLRAQLGI VSQEPILFDC SIAENIAYGD NSRVVPHDEI VRAAKEANIH PFIETLPQKY NTRVGDKGTQ LSGGQKQRIA IARALIRQPR VLLLDEATSA LDTESEKVVQ EALDKAREGR TCIVIAHRLS TIQNADLIVV IENGKVKEHG THQQLLAQKG IYFSMVNIQA GTQNL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Abcb4 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ABCB4 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ABCB4 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4 (ABCB4))
    Autre désignation
    Abcb4 (ABCB4 Produits)
    Synonymes
    ABC21 Protein, GBD1 Protein, ICP3 Protein, MDR2 Protein, MDR2/3 Protein, MDR3 Protein, PFIC-3 Protein, PGY3 Protein, zgc:172149 Protein, Mdr2 Protein, Pgy-2 Protein, Pgy2 Protein, mdr-2 Protein, Pgy3 Protein, ABCB4a Protein, DEFB1 Protein, PGP2 Protein, PGY2 Protein, PGP3 Protein, ABCB4 Protein, RUNDC3B Protein, ATP binding cassette subfamily B member 4 Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 4 Protein, beta-defensin 1-like Protein, ATP-binding cassette, sub-family B (MDR/TAP), member 1B Protein, RUN domain-containing protein 3B Protein, ABCB4 Protein, abcb4 Protein, Abcb4 Protein, LOC100861170 Protein, Abcb1b Protein, LOC101122517 Protein
    Sujet
    Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi between hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:8106172, PubMed:7912658, PubMed:7592705, PubMed:7814632, PubMed:8725158, PubMed:9366571). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (By similarity). Required for proper phospholipid bile formation (PubMed:8106172). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:7814632, PubMed:8725158). May promote biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (PubMed:1990275). {ECO:0000250|UniProtKB:P21439, ECO:0000269|PubMed:1990275, ECO:0000269|PubMed:21820390, ECO:0000269|PubMed:7592705, ECO:0000269|PubMed:7814632, ECO:0000269|PubMed:7912658, ECO:0000269|PubMed:8106172, ECO:0000269|PubMed:8725158, ECO:0000269|PubMed:9366571}.
    Poids moléculaire
    32.3 kDa Including tag.
    UniProt
    P21440
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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