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THRB Protein (AA 1-461) (His tag)

Crystallography grade THRB Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
N° du produit ABIN3133927
  • Antigène Voir toutes THRB Protéines
    THRB (Thyroid Hormone Receptor, beta (THRB))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-461
    Origine
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Source
    • 7
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette THRB protéine est marqué à la His tag.
    Application
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    MTPNSMTENG LPAWDKQKPR PDRGQDWKLV GMSEACLHRK SHVERRGALK NEQTSPHLIQ ATWTSSIFHL DPDDVNDQSI SSAQTFQTEE KKCKGYIPSY LDKDELCVVC GDKATGYHYR CITCEGCKGF FRRTIQKSLH PSYSCKYEGK CIIDKVTRNQ CQECRFKKCI YVGMATDLVL DDSKRLAKRK LIEENREKRR REELQKSIGH KPEPTDEEWE LIKTVTEAHV ATNAQGSHWK QKRKFLPEDI GQAPIVNAPE GGKVDLEAFS HFTKIITPAI TRVVDFAKKL PMFCELPCED QIILLKGCCM EIMSLRAAVR YDPDSETLTL NGEMAVTRGQ LKNGGLGVVS DAIFDLGMSL SSFNLDDTEV ALLQAVLLMS SDRPGLACVE RIEKYQDSFL LAFEHYINYR KHHVTHFWPK LLMKVTDLRM IGACHASRFL HMKVECPTEL FPPLFLEVFE D
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Thrb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product THRB Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    THRB (Thyroid Hormone Receptor, beta (THRB))
    Autre désignation
    Thrb (THRB Produits)
    Synonymes
    C-ERBA-2 Protein, C-ERBA-BETA Protein, ERBA2 Protein, GRTH Protein, NR1A2 Protein, PRTH Protein, THR1 Protein, THRB1 Protein, THRB2 Protein, Nr1a2 Protein, T3R[b] Protein, T3Rbeta Protein, Thrb1 Protein, Thrb2 Protein, c-erbAbeta Protein, TR-beta Protein, TR[b]1 Protein, fj24f03 Protein, trb Protein, wu:fj24f03 Protein, CTR BETA 2 Protein, TR-BETA Protein, LOC734147 Protein, thrb Protein, THRB Protein, C-erba-beta Protein, RATT3REC Protein, T3rec Protein, TRbeta Protein, THR Protein, TRbeta1 Protein, thyroid hormone receptor beta Protein, thyroid hormone receptor, beta Protein, thyroid hormone receptor, beta S homeolog Protein, thyroid hormone receptor, beta L homeolog Protein, THRB Protein, Thrb Protein, thrb Protein, thrb.S Protein, thrb.L Protein
    Sujet
    Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.
    Poids moléculaire
    53.6 kDa Including tag.
    UniProt
    P37242
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound
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