p21 Protein (AA 2-159) (His tag)

N° du produit ABIN3133955

Détail du produit

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Type de proteíne: Recombinant

Attributs du protein: AA 2-159

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette p21 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: SNPGDVRPVP HRSKVCRCLF GPVDSEQLRR DCDALMAGCL QEARERWNFD FVTETPLEGN FVWERVRSLG LPKVYLSPGS RSRDDLGGDK RPSTSSALLQ GPAPEDHVAL SLSCTLVSER PEDSPGGPGT SQGRKRRQTS LTDFYHSKRR LVFCKRKP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cdkn1a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Autre désignation: Cdkn1a (CDKN1A Produits)

Synonymes: CCND1 Protein, CDKN1A Protein, CAP20 Protein, CDKN1 Protein, CIP1 Protein, MDA-6 Protein, P21 Protein, SDI1 Protein, WAF1 Protein, p21CIP1 Protein, CDKI Protein, Cdkn1 Protein, Waf1 Protein, mda6 Protein, p21Cip1 Protein, p21WAF Protein, Cip1 Protein, UV96 Protein, p21 Protein, cip1 Protein, p16Xic2 Protein, sdi1 Protein, waf1 Protein, cap20 Protein, cdkn1 Protein, mda-6 Protein, p21cip1 Protein, si:ch1073-48m5.2 Protein, KRAS2 Protein, p21ras Protein, cyclin D1 Protein, cyclin dependent kinase inhibitor 1A Protein, cyclin-dependent kinase inhibitor 1A (P21) Protein, cyclin-dependent kinase inhibitor 1A Protein, cyclin-dependent kinase inhibitor 1A L homeolog Protein, cyclin-dependent kinase inhibitor 1 Protein, KRAS proto-oncogene, GTPase Protein, CCND1 Protein, CDKN1A Protein, Cdkn1a Protein, cdkn1a.L Protein, cki1 Protein, cdkn1a Protein, KRAS Protein

Sujet: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (By similarity). {ECO:0000250, ECO:0000269|PubMed:25329316}.

Poids moléculaire: 18.6 kDa Including tag.

UniProt: P39689

Pathways: Signalisation p53, Signalisation PI3K-Akt, Cycle Cellulaire, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy