MAP3K1 Protein (AA 2-1493) (His tag)

N° du produit ABIN3134459

Détail du produit

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1493

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette MAP3K1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Séquence: AAAAGDRASS SGFPGAAAAS PEAGGGGGGG GALQGSGAPA AGAAGLLREP GSAGRERADW RRRQLRKVRS VELDQLPEQP LFLAAASPPC PSTSPSPEPA DAAAGASRFQ PAAGPPPPGA ASRCGSHSAE LAAARDSGAR SPAGAEPPSA AAPSGREMEN KETLKGLHKM EDRPEERMIR EKLKATCMPA WKHEWLERRN RRGPVVVKPI PIKGDGSEVN NLAAEPQGEG QAGSAAPAPK GRRSPSPGSS PSGRSVKPES PGVRRKRVSP VPFQSGRITP PRRAPSPDGF SPYSPEETSR RVNKVMRARL YLLQQIGPNS FLIGGDSPDN KYRVFIGPQN CSCGRGAFCI HLLFVMLRVF QLEPSDPMLW RKTLKNFEVE SLFQKYHSRR SSRIKAPSRN TIQKFVSRMS NSHTLSSSST STSSSENSIK DEEEQMCPIC LLGMLDEESL TVCEDGCRNK LHHHCMSIWA EECRRNREPL ICPLCRSKWR SHDFYSHELS SPVESPASLR AVQQPSSPQQ PVAGSQRRNQ ESSFNLTHFG TQQIPSAYKD LAEPWIQVFG MELVGCLFSR NWNVREMALR RLSHDVSGAL LLANGESTGN SGGGSGGSLS AGAASGSSQP SISGDVVEAC CSVLSIVCAD PVYKVYVAAL KTLRAMLVYT PCHSLAERIK LQRLLRPVVD TILVKCADAN SRTSQLSIST VLELCKGQAG ELAVGREILK AGSIGVGGVD YVLSCILGNQ AESNNWQELL GRLCLIDRLL LEFPAEFYPH IVSTDVSQAE PVEIRYKKLL SLLTFALQSI DNSHSMVGKL SRRIYLSSAR MVTAVPAVFS KLVTMLNASG STHFTRMRRR LMAIADEVEI AEVIQLGVED TVDGHQDSLQ AVAPTSCLEN SSLEHTVHRE KTGKGLSATR LSASSEDISD RLAGVSVGLP SSTTTEQPKP AVQTKGRPHS QCLNSSPLSH AQLMFPAPSA PCSSAPSVPD ISKHRPQAFV PCKIPSASPQ TQRKFSLQFQ RNCSEHRDSD QLSPVFTQSR PPPSSNIHRP KPSRPVPGST SKLGDATKSS MTLDLGSASR CDDSFGGGGN SGNAVIPSDE TVFTPVEDKC RLDVNTELNS SIEDLLEASM PSSDTTVTFK SEVAVLSPEK AENDDTYKDD VNHNQKCKEK MEAEEEEALA IAMAMSASQD ALPIVPQLQV ENGEDIIIIQ QDTPETLPGH TKAKQPYRED AEWLKGQQIG LGAFSSCYQA QDVGTGTLMA VKQVTYVRNT SSEQEEVVEA LREEIRMMGH LNHPNIIRML GATCEKSNYN LFIEWMAGGS VAHLLSKYGA FKESVVINYT EQLLRGLSYL HENQIIHRDV KGANLLIDST GQRLRIADFG AAARLASKGT GAGEFQGQLL GTIAFMAPEV LRGQQYGRSC DVWSVGCAII EMACAKPPWN AEKHSNHLAL IFKIASATTA PSIPSHLSPG LRDVAVRCLE LQPQDRPPSR ELLKHPVFRT TW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Map3k1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Autre désignation: Map3k1 (MAP3K1 Produits)

Synonymes: MAP3K1 Protein, Mekk1 Protein, MAPKKK1 Protein, MEKK1 Protein, Mekk Protein, ARAKIN Protein, ATMEKK1 Protein, MAPK/ERK kinase kinase 1 Protein, MAPKKK8 Protein, T15F16.5 Protein, T15F16_5 Protein, MEKK Protein, MEKK 1 Protein, SRXY6 Protein, mitogen-activated protein kinase kinase kinase 1 Protein, mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase Protein, MAPK/ERK kinase kinase 1 Protein, MAP3K1 Protein, map3k1 Protein, Map3k1 Protein, MEKK1 Protein

Sujet: Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway. {ECO:0000269|PubMed:14500727}.

Poids moléculaire: 162.1 kDa Including tag.

UniProt: P53349

Pathways: Signalisation MAPK, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades