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COPS2 Protein (AA 1-443) (His tag)

Crystallography grade COPS2 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3134573
  • Antigène Voir toutes COPS2 Protéines
    COPS2 (COP9 Constitutive Photomorphogenic Homolog Subunit 2 (Arabidopsis) (COPS2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-443
    Origine
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Source
    • 7
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette COPS2 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MSDMEDDFMC DDEEDYDLEY SEDSNSEPNV DLENQYYNSK ALKEDDPKAA LSSFQKVLEL EGEKGEWGFK ALKQMIKINF KLTNFPEMMN RYKQLLTYIR SAVTRNYSEK SINSILDYIS TSKQMDLLQE FYETTLEALK DAKNDRLWFK TNTKLGKLYL EREEYGKLQK ILRQLHQSCQ TDDGEDDLKK GTQLLEIYAL EIQMYTAQKN NKKLKALYEQ SLHIKSAIPH PLIMGVIREC GGKMHLREGE FEKAHTDFFE AFKNYDESGS PRRTTCLKYL VLANMLMKSG INPFDSQEAK PYKNDPEILA MTNLVSAYQN NDITEFEKIL KTNHSNIMDD PFIREHIEEL LRNIRTQVLI KLIKPYTRIH IPFISKELNI DVADVESLLV QCILDNTIHG RIDQVNQLLE LDHQKRGGAR YTALDKWTNQ LNSLNQAVVS KLA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cops2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product COPS2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    COPS2 (COP9 Constitutive Photomorphogenic Homolog Subunit 2 (Arabidopsis) (COPS2))
    Autre désignation
    Cops2 (COPS2 Produits)
    Synonymes
    COPS2 Protein, ALIEN Protein, CSN2 Protein, SGN2 Protein, TRIP15 Protein, wu:fc05f05 Protein, wu:fc06g10 Protein, wu:fc60f04 Protein, wu:fl94f12 Protein, zgc:86624 Protein, csn2 Protein, Tb03.48O8.600 Protein, Trip15 Protein, AI315723 Protein, C85265 Protein, Csn2 Protein, Sgn2 Protein, alien-like Protein, ATCSN2 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 12 Protein, COP12 Protein, FUSCA 12 Protein, T20P8.4 Protein, T20P8_4 Protein, COP9 signalosome subunit 2 Protein, COP9 signalosome subunit 2 L homeolog Protein, COP9 signalosome complex subunit 2 Protein, COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) Protein, proteasome family protein Protein, COPS2 Protein, cops2 Protein, cops2.L Protein, Tb927.3.2320 Protein, AOR_1_576084 Protein, PTRG_07106 Protein, LOC100283498 Protein, Cops2 Protein, csn-2 Protein, FUS12 Protein
    Sujet
    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1. {ECO:0000269|PubMed:11967155, ECO:0000269|PubMed:12522100, ECO:0000269|PubMed:12972599}.
    Poids moléculaire
    52.6 kDa Including tag.
    UniProt
    P61202
    Pathways
    Cycle Cellulaire
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