RAD54L Protein (AA 1-747) (His tag)
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- Antigène Voir toutes RAD54L Protéines
- RAD54L (RAD54-Like (RAD54L))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-747
- Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette RAD54L protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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MRRSLAPSQL ARRKPEDRSS DDEDWQPGTV TPKKRKSSSE TQVQECFLSP FRKPLTQLLN RPPCLDSSQH EAFIRSILSK PFKVPIPNYQ GPLGSRALGL KRAGVRRALH DPLEEGALVL YEPPPLSAHD QLKLDKEKLP VHVVVDPILS KVLRPHQREG VKFLWECVTS RRIPGSHGCI MADEMGLGKT LQCITLMWTL LRQSPECKPE IEKAVVVSPS SLVKNWYNEV EKWLGGRIQP LAIDGGSKDE IDRKLEGFMN QRGARVPSPI LIISYETFRL HVGVLKKGNV GLVICDEGHR LKNSENQTYQ ALDSLNTSRR VLISGTPIQN DLLEYFSLVH FVNSGILGTA HEFKKHFELP ILKSRDAAAS EADRQRGEER LRELIGIVNR CLIRRTSDIL SKYLPVKIEQ VVCCRLTPLQ TELYKRFLRQ AKPEEELREG KMSVSSLSSI TSLKKLCNHP ALIYDKCVAE EDGFEGTLGI FPPGYNSKAV EPQLSGKMLV LDYILAVTRS RSSDKVVLVS NYTQTLDLFE KLCRVRRYLY VRLDGTMSIK KRAKVVERFN SPSSPDFVFM LSSKAGGCGL NLIGANRLVM FDPDWNPAND EQAMARVWRD GQKKICYIYR LLSAGTIEEK IFQRQSHKKA LSSCVVDEEQ DVERHFSLGE LKELFTLDEA SLSDTHDRLH CRRCVNNRQV WPPPDGSDCT SDLAQWNHST DKRGLQDEVL QAAWDASSTA ITFVFHQRSH EEQRGLH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Rad54l Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product RAD54L Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- RAD54L (RAD54-Like (RAD54L))
- Autre désignation
- Rad54l (RAD54L Produits)
- Synonymes
- HR54 Protein, RAD54A Protein, hHR54 Protein, hRAD54 Protein, RAD54 Protein, zgc:56289 Protein, rad54l Protein, MGC69368 Protein, GdRAD54 Protein, RAD54-like Protein, RAD54L Protein, RAD54 like Protein, RAD54 like (S. cerevisiae) Protein, RAD54-like (S. cerevisiae) Protein, RAD54L Protein, Rad54l Protein, rad54l Protein
- Sujet
- Involved in DNA repair and mitotic recombination. Functions in the recombinational DNA repair (RAD52) pathway. Dissociates RAD51 from nucleoprotein filaments formed on dsDNA. Could be involved in the turnover of RAD51 protein-dsDNA filaments (By similarity). Deficient mice also show significantly shorter telomeres than wild-type controls, indicating that the protein activity plays an essential role in telomere length maintenance in mammals. Deficiency also resulted in an increased frequency of end-to-end chromosome fusions involving telomeres compared to the controls, suggesting a putative role in telomere capping. Non-homologous end joining (NHEJ) and homologous recombination (HR) represent the two major pathways of DNA double-strand break (DSB) repair in eukaryotic cells. LIG4 and RAD54L cooperate to support cellular proliferation, repair spontaneous DSBs, and prevent chromosome and single chromatid aberrations. {ECO:0000250, ECO:0000269|PubMed:10209103, ECO:0000269|PubMed:10757799, ECO:0000269|PubMed:12218123, ECO:0000269|PubMed:12531026, ECO:0000269|PubMed:12548566, ECO:0000269|PubMed:12897131, ECO:0000269|PubMed:15175260, ECO:0000269|PubMed:9108475}.
- Poids moléculaire
- 85.6 kDa Including tag.
- UniProt
- P70270
- Pathways
- Réparation de l'ADN
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