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KCNQ1 Protein (AA 352-668) (His tag)

Crystallography grade KCNQ1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3134697
  • Antigène Voir toutes KCNQ1 Protéines
    KCNQ1 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 1 (KCNQ1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 352-668
    Origine
    • 2
    • 1
    Souris
    Source
    • 3
    Cellules d'insectes
    Purification/Conjugué
    Cette KCNQ1 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    LKVQQKQRQK HFNRQIPAAA SLIQTAWRCY AAENPDSATW KIYVRKPARS HTLLSPSPKP KKSVMVKKKK FKLDKDNGMS PGEKMFNVPH ITYDPPEDRR PDHFSIDGYD SSVRKSPTLL EVSTPHFLRT NSFAEDLDLE GETLLTPITH VSQLRDHHRA TIKVIRRMQY FVAKKKFQQA RKPYDVRDVI EQYSQGHLNL MVRIKELQRR LDQSIGKPSL FIPISEKSKD RGSNTIGARL NRVEDKVTQL DQRLVIITDM LHQLLSMQQG GPTCNSRSQV VASNEGGSIN PELFLPSNSL PTYEQLTVPQ TGPDEGS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Kcnq1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product KCNQ1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    KCNQ1 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 1 (KCNQ1))
    Autre désignation
    Kcnq1 (KCNQ1 Produits)
    Synonymes
    ATFB1 Protein, ATFB3 Protein, JLNS1 Protein, KCNA8 Protein, KCNA9 Protein, KVLQT1 Protein, Kv1.9 Protein, Kv7.1 Protein, LQT Protein, LQT1 Protein, RWS Protein, SQT2 Protein, WRS Protein, CG12215 Protein, CG12915 Protein, CG33135 Protein, DKCNQ Protein, Dmel\\CG33135 Protein, dKCNQ Protein, kcnq1 Protein, KCNQ1 Protein, kqt-3 Protein, kv7.1 Protein, Kvlqt1 Protein, KvLQT-1 Protein, kcnq1-A Protein, xkvlqt1 Protein, zgc:158384 Protein, AW559127 Protein, Kcna9 Protein, KvLQT1 Protein, potassium voltage-gated channel subfamily Q member 1 Protein, KCNQ potassium channel Protein, potassium voltage-gated channel, KQT-like subfamily, member 1 Protein, potassium channel, voltage gated KQT-like subfamily Q, member 1 Protein, voltage gated potassium channel subunit Protein, potassium channel, voltage gated KQT-like subfamily Q, member 1 L homeolog Protein, potassium voltage-gated channel, subfamily Q, member 1 Protein, KCNQ1 Protein, KCNQ Protein, kcnq1 Protein, Kcnq1 Protein, kcnq1.L Protein
    Sujet
    Potassium channel that plays an important role in a number of tissues, including heart, inner ear, stomach and colon (By similarity) (PubMed:16314573, PubMed:11120752, PubMed:15004216). Associates with KCNE beta subunits that modulates current kinetics (By similarity) (PubMed:17597584, PubMed:15004216). Induces a voltage-dependent by rapidly activating and slowly deactivating potassium-selective outward current (By similarity) (PubMed:8900282). Promotes also a delayed voltage activated potassium current showing outward rectification characteristic (By similarity). During beta-adrenergic receptor stimulation participates in cardiac repolarization by associating with KCNE1 to form the I(Ks) cardiac potassium current that increases the amplitude and slows down the activation kinetics of outward potassium current I(Ks) (By similarity) (PubMed:15004216, PubMed:17597584). Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current (By similarity). When associated with KCNE3, forms the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions (By similarity). This interaction with KCNE3 is reduced by 17beta-estradiol, resulting in the reduction of currents (By similarity). During conditions of increased substrate load, maintains the driving force for proximal tubular and intestinal sodium ions absorption, gastric acid secretion, and cAMP-induced jejunal chloride ions secretion (PubMed:16314573). Allows the provision of potassium ions to the luminal membrane of the secretory canaliculus in the resting state as well as during stimulated acid secretion (PubMed:19491250). When associated with KCNE2, forms an heterooligomer complex leading to currents with an apparently instantaneous activation, a rapid deactivation process and a linear current-voltage relationship and decreases the amplitude of the outward current (By similarity). When associated with KCNE4, inhibits voltage-gated potassium channel activity (By similarity). When associated with KCNE5, this complex only conducts current upon strong and continued depolarization (By similarity). Also forms a heterotetramer with KCNQ5, has a voltage-gated potassium channel activity (By similarity). Binds with phosphatidylinositol 4,5-bisphosphate (By similarity). {ECO:0000250|UniProtKB:P51787, ECO:0000250|UniProtKB:Q9Z0N7, ECO:0000269|PubMed:11120752, ECO:0000269|PubMed:15004216, ECO:0000269|PubMed:16314573, ECO:0000269|PubMed:17597584, ECO:0000269|PubMed:19491250, ECO:0000269|PubMed:8900282}.
    Poids moléculaire
    37.1 kDa Including tag.
    UniProt
    P97414
    Pathways
    Negative Regulation of Hormone Secretion, Sensory Perception of Sound
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