MYO3B Protein (AA 1-1305) (Strep Tag)

N° du produit ABIN3134927

Détail du produit

Antigène: MYO3B (Myosin IIIB (MYO3B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1305

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MYO3B protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MMLGLESLPD PMETWEIIET IGKGTYGKVY KVANKRDGSL AAVKVLDPVS DMDEEIEAEY NILQFLPSHP NVVKFYGMFY KADRCVGGQL WLVLELCNGG SVTELVKGLL RCGKRLDEAV ISYILYGALL GLQHLHCHRI IHRDVKGNNI LLTTEGGVKL VDFGVSAQLT STRLRRNTSV GTPFWMAPEV IACEQQYDSS YDARCDVWSL GITAIELGDG DPPLFEMHPV KMLFKIPRNP PPTLLHPDSW CEEFNHFISQ CLIKDFEKRP SVTHLLDHPF IKGTQGKVLC LQKQLAKVLQ DQKHRNPVAK TRHERMHTGR PHRVEDAGKC CLEDDLVNLE VLDEDTIIYW LQKRYADALI YTYVGDILIA LNPFQNLSIY SPQFSRLYHG VKRSSNPPHI FASADNAYQC LVTFSKDQCI VISGESGSGK TESAHLIVQH LTFLGKADNQ TLRQKILQVN SLVEAFGNAR TAINDNSSRF GKYLEMMFTP TGAVMGARIS EYLLEKSRVI QQAAGEKNFH IFYYIYAGLY HQKKLAEFRL PEEKPPRYIA GETERVMQDI TSKESYRTQF EAIQHCFKII GFADKEVHSV YRILAGILNI GSIEFAAISS QHQTDKSEVP NPEALENAAC VLCISSEELQ EALTSHCVVT RGETIVRANT VDRAEDVRDA MSKALYGRLF SWIVNRINTL LQPDKNICSA EDRMNVGILD IFGFEDFQRN SFEQLCINIA NEQIQYYFNQ HVFALEQMEY KNEGVDAVLV QYEDNRPLLD MFLQKPLGLL ALLDEESRFP QGTDQTLVDK FEDNLRCKFF WRPKGVELCF GIQHYAGPVL YDASGVLEKN RDTLPADVVV VLRTSENKLL QQLFSIPLTK TGNLAQTRAK ITASSRSLPP HFSAGRAKVD TLEVIRHPEE TTNMKRQTMA SYFRYSLMDL LSKMVVGQPH FIRCIKPNDD RKALQFSQDR VLAQLRSTGI LETVSIRRQG YSHRIFFEEF VKRYYYLAFR AHQTPPANKE SCVAILEKSR LDHWVLGKTK VFLKYYHVEQ LNLLLREVMG RVVMLQAYTK GWLGARRYKR AKEKREKGAI TIQSAWRGYD ARRKLKQRSR RRSESEAHIH TVLQTTPDQK YCPDSGGESN RGHEETSRNC PAEADTDGHP QAQSPPTGCD VTSGHADTAA GYTVAELSVA GTDVSPSLVY HTASAHQRLS PCEDSLKPGS EEGLSQKQRA PRRRCQQPKM LSSPEDTMYY NQLNGTLEYQ GSQRKPRKLG QIKVLDGEDQ YYKCLSPGAC APEETHSVHP FFFSSSPRED PFAQH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MYO3B (Myosin IIIB (MYO3B))

Autre désignation: Myo3b (MYO3B Produits)

Synonymes: RGD1560313 Protein, A430065P19Rik Protein, myosin IIIB Protein, myosin-IIIb Protein, myosin 3b Protein, Myo3b Protein, MYO3B Protein, myo3b Protein, LOC100063200 Protein, LOC100542076 Protein, CpipJ_CPIJ008894 Protein

Sujet: Myosin-IIIb (EC 2.7.11.1),FUNCTION: Probable actin-based motor with a protein kinase activity (By similarity). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646). Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607). {ECO:0000250|UniProtKB:Q8WXR4, ECO:0000269|PubMed:22264607, ECO:0000269|PubMed:26754646}.

Poids moléculaire: 148.1 kDa

UniProt: Q1EG27