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MCM9 Protein (AA 1-1134) (His tag)

Crystallography grade MCM9 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3134936
  • Antigène Voir toutes MCM9 Protéines
    MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1134
    Origine
    Souris
    Source
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette MCM9 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MNSEQVTLVG QVFESYVSEY HKNDILLILK ERDEDAHYPV VVNAMSLFET NMEIGDYFTV FPNEVLTVFD SALRRSALAI LQSLPETEGL SMKQNLHARI SGLPVCPELV REHIPKTKDV GHFLSVTGTV IRTSLVKVLE FERDYMCNKC KHVFMVEADF EQYYTFSRPS SCPSLASCDS SKFSCLSDLS SSPARCRDYQ EIKIQEQVQR LSVGSIPRSM KVILEDDLVD SCKSGDDLTI YGVVMQRWKP FQRDVRCEVE IVLKANYVQV NNEQSSGMVM DEDTRKEFED FWEHYKSDPF AGRNEILASL CPQVFGMYLV KLAVAMVLAG GIQRTDAAGT RVRGESHLLL VGDPGTGKSQ FLKYAAKITP RSVLTTGIGS TSAGLTVTAV KDSGEWNLEA GALVLADAGL CCIDEFNSLK EHDRTSIHEA MEQQTISVAK AGLVCKLNTR TTILAATNPK GQYDPKESVS VNIALGSPLL SRFDLVLVLL DTRNEDWDRI ISSFILENKG YPSKSENLWS MEKMKTYFCL IRNLHPTLSE VSNQVLLRYY QMQRQSDSRN AARTTIRLLE SLIRLAEAHA RLMFRSAVTL EDAVTAVSVM ESSMQGGALL GGVNALHTSF PENPRAQYQR QCELILEKLE LQGLLQEELR RLERLQNESV HQCQSHSLEE EVAPGSCRND PRDKPRLRTS TQQEQSCSWS STERSGADSP PGPGLNRPTS CNNSAENRDG RGDGLDWLDP TSSPEIAPES TIVSPNVKTT EKNVNLKISN NKSQGKEKHG PQQRSKLLEA GHLPSSGAMN APLRSHGVKR TKASQAVVVS EAGRGDEEDS VPRRLPKLLK EGSQNVCRST TRVRPLPPTV PLSLSIPSPG SGKRSGTPKR KRRKSAQVEE PEPEGMETPT VKLAKFTFKQ KTKLTHSPEG QGPIPPSASE IAVDSSKIPQ QRTRREAAVP VVAPGKSTST SGDRCSDQLH GKTKELSRQP PDSNPPREER EQGPKRRVIQ PKPELGNQAG HSHLACEKDR KEGVSCGNKS SKVHAGTIAR LASFSFTSPS ESKSESLPPE RKDSRDSRDS RDSRDRCHSP PATTAPVLGQ QRQTFQLQQP TERANLSTLS LFTLSELDDE ALDFDWEEEM RKKP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mcm9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MCM9 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MCM9 (Minichromosome Maintenance Deficient 9 (MCM9))
    Autre désignation
    Mcm9 (MCM9 Produits)
    Synonymes
    C6orf61 Protein, MCMDC1 Protein, dJ329L24.1 Protein, dJ329L24.3 Protein, 9030408O17Rik Protein, Gm235 Protein, Mcmdc1 Protein, RGD1560557 Protein, mcm9 Protein, minichromosome maintenance 9 homologous recombination repair factor Protein, minichromosome maintenance 9 homologous recombination repair factor L homeolog Protein, MCM9 Protein, Mcm9 Protein, mcm9.L Protein
    Sujet
    Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. {ECO:0000269|PubMed:21987787, ECO:0000269|PubMed:22771120}.
    Poids moléculaire
    126.8 kDa Including tag.
    UniProt
    Q2KHI9
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