STK11IP Protein (AA 1-1072) (Strep Tag)

N° du produit ABIN3134960

Détail du produit

Antigène: STK11IP (serine/threonine Kinase 11 Interacting Protein (STK11IP))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1072

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette STK11IP protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MTTAPRDSVV WKLAGLLRES GDAVLSGCST LSLLTATLQQ LNRVFELYLG PWGPGQTGFV ALPSHPADSP VILQLQFLFD VLQKTLSLKL VHIPGVGLPG PIKIFPFKSL RQLELRGVPI HSLCGLRGIY SQLESLVCNR SIQALEELLS ACGGDLCSAL PWLALLSADF SYNALRSLDS SLRLLSALRF LNLSHNHLQD CKGFLMDLCE LYHLDISYNH LRLVPRVGPS GAALGTLILR ANELRSLQGL EQLKNLRHLD VAYNLLEGHT ELAPLWLLAE LRKLYLEGNP LWFHPAHRAA TAQYLSPRAR DAAHGFLLDG KVLSLKDLQQ TSDSSGLGPV IQPLSWPVGS TTETSGGPEL SDSLSSGGIV AQAPLRKVKS RVRVRRASIS EPSDTDPELR TLDPSPAGWF VQQHRELELL ASFRERFGCD WLQYRSHLET MGSSPLSTTK TPALSTPPLD VQNLETVCSP PAIEDDTKES PEKVSEEGRV EPEPQEEERE EQDKEEGSRE DLEEEEEQEQ KAVEAELCRP MLVCPLQGLG GVQGKECFLR VTSAHLFEVE LQAARTLERL ELQSLVAAEL ESETETQGEP VSEGSGPFPG APVLVLRFSY ICPDRQLRRY AVLEPEAREA VQELLAVLTP FTSVKEQQPG ANKDPQGARF QCLRCSCEFK PEESRLGLES DEGWKPLFQN TESPVVCPNC GSDHVVLLAV SGEVPNREQN QEEQSADSAC DLADHGGCPS RPDGIPPQTS ISHDRSSWSL SPSPGCSGFR SVDHRLRLFL DVEVFSDSEE EFQCCIKVPV VLAGHTEEFP CLVVVSNHML YLLKVLGAIC GPPASWLQPT LAIALQDLSG MELGLAGQSL RLEWAAGTGS CVLLPRDARQ CRAFLEELTG VLQSLPRTQR NCISATEEEV TPQHRLWPLL GKDPSAEVPQ FFYLRAFLAE GSSTCPVSLL LTLSTLYLLD EDPGGSHAES PLPVVSDETS EQPASLGPGP SLQVREQQPL SCLSSVQLYR TSPLDLRLIF YDEVSRLESF WALHVVCGEQ LTALLAWIRE PWEELFSIGL RTVTQEALDL DR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: STK11IP (serine/threonine Kinase 11 Interacting Protein (STK11IP))

Autre désignation: Stk11ip (STK11IP Produits)

Synonymes: LIP1 Protein, LKB1IP Protein, STK11IP1 Protein, 1200014D22Rik Protein, BB131189 Protein, serine/threonine kinase 11 interacting protein Protein, serine/threonine kinase 11 interacting protein L homeolog Protein, STK11IP Protein, Stk11ip Protein, stk11ip.L Protein

Sujet: Serine/threonine-protein kinase 11-interacting protein (LKB1-interacting protein 1),FUNCTION: May regulate STK11/LKB1 function by controlling its subcellular localization. {ECO:0000250}.

Poids moléculaire: 118.0 kDa

UniProt: Q3TAA7