RIC8A Protein (AA 1-530) (Strep Tag)

N° du produit ABIN3134973

Détail du produit

Antigène: RIC8A (Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-530

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette RIC8A protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEPRAVADAL ETGEEDAVTE ALRSFNREHS QSFTFDDAQQ EDRKRLAKLL VSVLEQGLSP KHRVTWLQTI RILSRDRSCL DSFASRQSLH ALACYADITV SEEPIPQSPD MDVLLESLKC LCNLVLSSPT AQMLAAEARL VVRLAERVGL YRKRSYPHEV QFFDLRLLFL LTALRTDVRQ QLFQELHGVR LLTDALELTL GVAPKENPPV MLPAQETERA MEILKVLFNI TFDSVKREVD EEDAALYRYL GTLLRHCVMV EAAGDRTEEF HGHTVNLLGN LPLKCLDVLL ALELHEGSLE FMGVNMDVIS ALLAFLEKRL HQTHRLKECV APVLNVLTEC ARMHRPARKF LKAQVLPPLR DVRTRPEVGD LLRNKLVRLM THLDTDVKRV AAEFLFVLCS ESVPRFIKYT GYGNAAGLLA ARGLMAGGRP EGQYSEDEDT DTEEYREAKA SINPVTGRVE EKPPNPMEGM TEEQKEHEAM KLVNMFDKLS RHRVIQPMGM SPRGHLTSLQ DAMCETMEGQ LSSDPDSDPD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RIC8A (Resistance To Inhibitors of Cholinesterase 8 Homolog A (C. Elegans) (RIC8A))

Autre désignation: Ric8a (RIC8A Produits)

Synonymes: RIC8 Protein, AI114950 Protein, RIC-8 Protein, Ric8a Protein, Ric-8A Protein, Ric8 Protein, ric-8 Protein, ric8 Protein, synembryn Protein, xtric-8 Protein, zgc:92294 Protein, Syn Protein, ric8a Protein, ric8ab Protein, CG15797 Protein, DmRic8 Protein, Dmel\\CG15797 Protein, Ric-8 Protein, l(1)G0397 Protein, ric-8a Protein, ricc8aa Protein, fj97c10 Protein, synbl Protein, wu:fj97c10 Protein, RIC8 guanine nucleotide exchange factor A Protein, microRNA 6743 Protein, synembryn-A Protein, RIC8 guanine nucleotide exchange factor A L homeolog Protein, CG15797 gene product from transcript CG15797-RB Protein, synembryn Protein, RIC8 guanine nucleotide exchange factor A S homeolog Protein, Synembryn Protein, RIC8 guanine nucleotide exchange factor B Protein, RIC8A Protein, Ric8a Protein, ric8a Protein, MIR6743 Protein, Tsp_08768 Protein, ric8a.L Protein, LOC411841 Protein, ric8a.S Protein, ric-8 Protein, ric8b Protein

Sujet: Synembryn-A (Protein Ric-8A),FUNCTION: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation (By similarity). {ECO:0000250}.

Poids moléculaire: 59.8 kDa

UniProt: Q3TIR3

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Asymmetric Protein Localization