DENND1B Protein (AA 1-766) (Strep Tag)

N° du produit ABIN3135010

Détail du produit

Antigène: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Type de proteíne: Recombinant

Attributs du protein: AA 1-766

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DENND1B protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MDRRTRENPE RTFDLVLKVK CHASENEDPE VLWKFPEDFG DQEVLQSVPK FCFPFDVERV SQNQVGQHFT FVLTDMESKQ RFGFCRLTSG GRICLCILSY LPWFEVYYKL LNTLADYLAK ELEEDLNETL KSLYNHPVPK ANTPVNLSVH SCFITPDITG LPTIPESRNL TEYFVAVDVN NMLRLYASML HERRIIITSS KLSTLTACLH GSAALLYPMY WQHIYIPVLP PHLLDYCCAP MPYLIGIHSS LIERVKNKSL EDVVVLNVDT NTLESPFNDL SSLPSDVVSA LKNKLKKQST ATGDGVARAF LRAQAALFGS YRDALRYKPG EPITFCEESF VKHRSSVMKQ FLETAVNLQL FKQFIDGRLA KLNAGRGFSD IFEEEITSGG FCGGSPRSYQ QWVYTVKKGG ALFNTAVTKA TPAVRTAYKF AKSHARLGLK EVKSRLKHKD NEEEYGTCSG LVQYTPVYTL HNEKGENREK HKLSQTHLKR PHKSLDGTLY DDDDDDDDIE RASKISSEDG EETRAYFYES DDSVEAQVKA PYSGEMDLLG EILDTLSTHS SDQGKLAPAK SLDFFRSMDD IDYKPTNKSN APSENNLALL CASGDQGEWN LGQDDSALHG RQLPPSPRKR VSSGGLTESL FILKEESREK PLCADSVSGP TVVGKPAPTS GLRSQPAAPE ASQTERGRAE VKQTPGQAPL QSEDLSVPGP GSRQSTFVPW EKAGKEDTKP SKDVGLLQEV VSLCHMSCDF QQDLNISEES RSGNQT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DENND1B (DENN/MADD Domain Containing 1B (DENND1B))

Autre désignation: Dennd1b (DENND1B Produits)

Synonymes: C1ORF18 Protein, C1orf218 Protein, FAM31B Protein, 4632404N19Rik Protein, 4930467M19Rik Protein, 6820401H01Rik Protein, F730008N07Rik Protein, Fam31b Protein, si:ch211-195i21.1 Protein, DENN domain containing 1B Protein, DENN/MADD domain containing 1B Protein, DENND1B Protein, dennd1b Protein, Dennd1b Protein

Sujet: DENN domain-containing protein 1B (Connecdenn 2),FUNCTION: Guanine nucleotide exchange factor (GEF) for RAB35 that acts as a regulator of T-cell receptor (TCR) internalization in TH2 cells. Acts by promoting the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Plays a role in clathrin-mediated endocytosis. Controls cytokine production in TH2 lymphocytes by controlling the rate of TCR internalization and routing to endosomes: acts by mediating clathrin-mediated endocytosis of TCR via its interaction with the adapter protein complex 2 (AP-2) and GEF activity. Dysregulation leads to impaired TCR down-modulation and recycling, affecting cytokine production in TH2 cells. {ECO:0000269|PubMed:26774822}.

Poids moléculaire: 85.5 kDa

UniProt: Q3U1T9