AHRR Protein (AA 1-701) (Strep Tag)

N° du produit ABIN3135011

Détail du produit

Antigène: AHRR (Aryl-Hydrocarbon Receptor Repressor (AHRR))

Type de proteíne: Recombinant

Attributs du protein: AA 1-701

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette AHRR protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MMIPSGECTY AGRKRRKPIQ KRRLTMGAEK SNPSKRHRDR LNTELDHLAS LLPFSPDIIS KLDKLSVLRL SVSYLRVKSF FQALQETCVW SAPALSPEEH SYRGFPVQEG RLLLESLNGF ALVVSAEGMI FYASATIVDY LGFHQTDVMH QNIYDYIHVD DRQDFCRQLH WAMDPPQVVF GQSPHADTDN TVLGKLLRAQ EGGKGLPSEY SAFLTRCFIC RVRCLLDSTS GFLTMQFQGK LKFLFGQKKK TPSGTALPPR LSLFCIVAPV LPSVTEMKMK STFLKAKHRA DIVVTMDSRA KAVTSLCESE LHPKLNYLAG KSNGENGISL FRGQTDRSHW ARALARSSCL CLRGGPDLLD PKGTSGDREE EDQKHILRRS PGAWGQREMH KYSYGLETPV HLRHLNWSTE QRSQESTTKL TRQPSKNEPS TCLVPHGSCV PYPGSQGMLS ASNMASFRDS LDHPTGAYCS QMNRPLSDIH QGQVDPSTCH ISQGSLGSRI PLTGMQRFTA RGFSTEDAKL PSLPVTIGTP CNPVLSLDVP IKMENESGSQ DIVEASTTSC LWLGTSDMAR GHLVGFPARM HLKTEPDYRQ QACTPHLGHG MLGTNPYSRD TVGSCREHAP LYSAHCTCLD PEPPHHLFMC SHSESQHPSL DQDCRAPIVK REPLDSPSWA APGQVTVPRM FPKSASKTVI PSKGSDGIFL P
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: AHRR (Aryl-Hydrocarbon Receptor Repressor (AHRR))

Autre désignation: Ahrr (AHRR Produits)

Synonymes: mKIAA1234 Protein, AHH Protein, AHHR Protein, bHLHe77 Protein, ahh Protein, ahhr Protein, bhlhe77 Protein, ahrr2 Protein, aryl-hydrocarbon receptor repressor Protein, aryl hydrocarbon receptor repressor-like Protein, uncharacterized LOC723045 Protein, aryl-hydrocarbon receptor repressor L homeolog Protein, aryl hydrocarbon receptor repressor Protein, aryl-hydrocarbon receptor repressor b Protein, Ahrr Protein, AHRR Protein, LOC529500 Protein, LOC723045 Protein, ahrr.L Protein, LOC100455869 Protein, ahrr Protein, LOC100136487 Protein, ahrrb Protein

Sujet: Aryl hydrocarbon receptor repressor (AhR repressor) (AhRR),FUNCTION: Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site. {ECO:0000269|PubMed:17949687, ECO:0000269|PubMed:9887096}.

Poids moléculaire: 77.7 kDa

UniProt: Q3U1U7

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha