CAZIP Protein (AA 1-1353) (Strep Tag)

N° du produit ABIN3135047

Détail du produit

Antigène: CAZIP (MTUS2) (Microtubule-associated Tumor Suppressor Candidate 2 (MTUS2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1353

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CAZIP protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSVPLAPKKS CFGQLRDHRE GAKNNNESIL RTGDTNANQI MLEVSSSCDE AKSRDLDDEL GNSNLSRPQY HSHFQKEPLH LQGFGKGSQA GSTSQRESQA SLTVHRQLSE EHAVKRGALQ APQCVQGPSL SSWRNAVGQA SPEASAKKDA EIPRHIPKDK LAKTLDNEEL KRASSCSAAA GSVPPTDLQP VQLDTLGPQD HVPARGEGPQ RTPASHSPGK GFSPGEGTSE GNSVYLPKPS TSEAKGSSPS DTKMEGPHGL DVYNERITHA ELTPSSASAS KENPGLRHPE VCLGQGTGKS KVELKSVQPR NEEGLTSAQA QGPGCHEERS MSPVERQELL EKAYREATSQ GNSSHRQLGV RRGSSLEEMT GVSAGVEGSQ QATPTLSAAP AGEAGTRLTG KMSAGVGRMA RETASGQTAP DVGQAAPVRR DPTESVPSEV SGEERRLGSG NSGSTKLLAS GPSAGGSRTD TSGLLSPRGS NLEARKGKEM VAENRNLLEN AAQTDNTPAG VDSAFSTPAP LLHPETTVNS SHHPTPPGSS SQELGVFSGD TGSPSVASPP TDGGQVLNTS PKVPDRTTCS SGIPKPPTHP KDTPSSQEAR EKLETEKMEE RAEAKPILMP KPKHVRPKII TYIRRNPQAL SQGDASLVPV GLPYAPPTCG MPLPQEEKAA SRDLQPSANM YEKLKPDLQK PRVFPSGLMV SGIKPPAHHF SQMSEKFLQE VADHPGKEEF CSPPYTHYEV PPTFYRSAML LKPQLGLGAM SRLPSTKSRI LIASQRSSAS AIHPPGSLTT AASFYGSDPS DLKKASNSNA AKASLPKSGL RPPGYSRLPA AKLAAFGFVR SSSISAVPSS QSLDSVQPEQ SRPVTRSTFG NEEQAPLKQA LPSKDTPKGA GRAAPASSSN ATTPRRSLLP APKSTSTPAG AKKELQKDPE AKKPAVSSPK RTASAATKPH SPGYPKQRTS APRNEFPPKP DLQAREAERQ LAQRLRDRCE WQARQLGLAR RELKKAIQGF DALAVSTKHF FGKSERALAK EKELSIELAN IRDEVAFNTA KCEKLQKEKE TLERRFEEEL RRLGWQQQAE VQELQERLQQ QFQAESARLQ AEHQDQLLRM RCQHQEQVED ITASHEAALL EMENNHTVAI TILQDDHDHK VQELMSTHEF EKKELEENFE KLRLTLQDQV DTLTFQSQSL RDRARRFEEA LRKTTEEQLE IALAPYQHLE EDMQSLKQVL EMKNQQIHLQ EKKIIELEKL VEKNIILEER IQVLQQQNED LKARIDQNTV VTRQLSEENA NLQEYVEKET QEKKRLSRTN EELLWKLQTG DPTSPIKLSP TSPVYRGSSS GPSSPARVST TPR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CAZIP (MTUS2) (Microtubule-associated Tumor Suppressor Candidate 2 (MTUS2))

Autre désignation: Mtus2 (MTUS2 Produits)

Synonymes: KIAA0774 Protein, DKFZp468L0812 Protein, CAZIP Protein, ICIS Protein, TIP150 Protein, 5730592G18Rik Protein, A730013O20Rik Protein, AU067634 Protein, C130038G02Rik Protein, Cazip Protein, Gm763 Protein, mKIAA0774 Protein, microtubule associated tumor suppressor candidate 2 Protein, microtubule associated scaffold protein 2 Protein, MTUS2 Protein, Mtus2 Protein

Sujet: Microtubule-associated tumor suppressor candidate 2 homolog (Cardiac zipper protein) (Microtubule plus-end tracking protein TIP150) (Tracking protein of 150 kDa),FUNCTION: Binds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip (By similarity). {ECO:0000250}.

Poids moléculaire: 147.4 kDa

UniProt: Q3UHD3