SNX27 Protein (AA 1-539) (Strep Tag)

N° du produit ABIN3135048

Détail du produit

Antigène: SNX27 (Sorting Nexin 27 (SNX27))

Type de proteíne: Recombinant

Attributs du protein: AA 1-539

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SNX27 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MADEDGEGIH PSAPHRNGGG GGGSGLHCAG NGGGGGGGPR VVRIVKSESG YGFNVRGQVS EGGQLRSING ELYAPLQHVS AVLPGGAADR AGVRKGDRIL EVNGVNVEGA THKQVVDLIR AGEKELILTV LSVPPHEADN LDPSDDSLGQ SFYDYTEKQA VPISVPTYKH VEQNGEKFVV YNVYMAGRQL CSKRYREFAI LHQNLKREFA NFTFPRLPGK WPFSLSEQQL DARRRGLEEY LEKVCSIRVI GESDIMQEFL SESDENYNGV SDVELRVALP DGTTVTVRVK KNSTTDQVYQ AIAAKVGMDS TTVNYFALFE VINHSFVRKL APNEFPHKLY VQNYTSAVPG TCLTIRKWLF TTEEEVLLND NDLAVTYFFH QAVDDVKKGY IKAEEKSYQL QKLHEQRKMV MYLNMLRTCE GYNEIIFPHC ACDSRRKGHV ITAISITHFK LHACTEEGQL ENQVIAFEWD EMQRWDTDEE GMAFCFEYAR GEKKPRWVKI FTPYFNYMHE CFERVFCELK WRKENIFQMA RSQQRDVAT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SNX27 (Sorting Nexin 27 (SNX27))

Autre désignation: Snx27 (SNX27 Produits)

Synonymes: MRT1 Protein, MY014 Protein, 5730552M22Rik Protein, ESTM45 Protein, ESTM47 Protein, R75405 Protein, mKIAA0488 Protein, Mrt1 Protein, sorting nexin family member 27 Protein, SNX27 Protein, Snx27 Protein

Sujet: Sorting nexin-27,FUNCTION: Involved in the retrograde transport from endosome to plasma membrane, a trafficking pathway that promotes the recycling of internalized transmembrane proteins. Following internalization, endocytosed transmembrane proteins are delivered to early endosomes and recycled to the plasma membrane instead of being degraded in lysosomes. SNX27 specifically binds and directs sorting of a subset of transmembrane proteins containing a PDZ-binding motif at the C-terminus: following interaction with target transmembrane proteins, associates with the retromer complex, preventing entry into the lysosomal pathway, and promotes retromer-tubule based plasma membrane recycling. SNX27 also binds with the WASH complex. Interacts with membranes containing phosphatidylinositol-3-phosphate (PtdIns(3P)). May participate in establishment of natural killer cell polarity. Recruits CYTIP to early endosomes. {ECO:0000269|PubMed:23524343}.

Poids moléculaire: 61.0 kDa

UniProt: Q3UHD6