ARAP1 Protein (AA 1-1452) (Strep Tag)

N° du produit ABIN3135141

Détail du produit

Antigène: ARAP1 (ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1452

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ARAP1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAEGYDAALS VAEWLRALHL EQYTALFEQH GLVWATECQG LSDAGLLDMG MHLPGHRRRI LAGLHRAHAP PVPLPRPAPR PVPMKRHIFR SPPVPVTPPE PPPTAGEDEG LPAAPPIPPR RSCLPPACFT PTSTAAPDPV LPPLPAKRHL VEPSVPPVPP RTGPPYPQAS LLAKEELLLP SVSPRSQPEP AETPSTLLPA FPQGPLQPPS PPPCPPVIPP KPPRLLPEFD DSDYDDVPEE GPGAPASVMT KEEPLPSRVP RAVRVASLLS EGEELSGDDS EDDDDHAYEG IPNGGWPTSG LNPPLRSLIP DLPLHPMDEL PGGPTPITPV IKAGWLDKNP PQGSYIYQKR WVRLDADYLR YFDSNKDAYS KRFVPVACIC RVAPIGDQKF EVITNNRTFA FRAESDVERN EWMQALQQAV VEHRARFRLS SASVLGVRGS EQPDRAGSLE LRGFKNKLYV AVTGDKVQLY KNLEEFHLGI GITFIDMNVG NVKEVDRRSF DLTTPYRIFS FSADSELEKE QWLEAMQGAI AEALSTSEVA ERIWAAAPNR FCADCGAAQP DWASINLCVV ICKRCAGEHR GLGAGVSKVR SLKMDRKVWT EALIQLFLHL GNGPGNHFWA ANVPPSEALE PSSSPGARRY HLEAKYREGK YRRYHPLFGN QEELDKALCA AVTTTDLAET QALLGCGAGV SCFSGDPAAP TPLALAEQAG QTLQMEFLRN NQSTEVPRLD SVKPLEKHYS VTLPTVSHSG FLYKTASAGK PLQDRRAREE FSRRWCVLSD GVLSYYENER AVTPNGEIRA SEIVCLAVSP LDTHGFEHTF EVYTEGERLY LFGLENAELA HEWVKCIAKA FVPPLAEDLL ARDFERLGRL PCKAGLSLQQ AQEGWFALTG SELRAVFPEG PWEEPLQLRK LQELSIQGDS ENQVLVLVER RRTLYIQGER RLDFMAWLGV IQKAAASLGD TLSEQQLGDS DIPVIVYRCV DYITQCGLTS EGIYRKCGQT SKTQRLLDSL RQDARSVHLK EGEQHVDDVS SALKRFLRDL PDGLFTRAQR LAWLEASEIE DEEEKISRYR ELLVHLPPVN RATVKALISH LYCVQCFSDT NQMNTHNLAI VFGPTLFQTD GQDYKAGKVV EDLINHYVVV FSVDEEELRK QREEVTAIVK MRVAGTASGT QHAGDFICTV YLEEKKVETE QHVKIPASMT AEELTLEILD RRNVSIREKD YWTCFEVNEK EEAERPLHFA EKVLPIVHGL GIDSHLVVKK YQSMEAMLLY LASRVGDTKH GMMKFREDRS LLGLGLPSGG FHDRYFILNS SCLRLYKEVR SQRPWSGAPE TSHRPEKEWP VKSLKVYLGV KKKLRPPTCW GFTVVHETEK HEKQQWYLCC DTQMELREWF ATFLSVQHDG LVWPSEPSRV SRAVPEVRMG SVSLIPLRGS ENEMRRSVAA FTADPLSLLR HV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARAP1 (ArfGAP with RhoGAP Domain, Ankyrin Repeat and PH Domain 1 (ARAP1))

Autre désignation: Arap1 (ARAP1 Produits)

Synonymes: CENTD2 Protein, ARAP1 Protein, Ci-ArfGAP-8 Protein, 2410002L19Rik Protein, Centd2 Protein, mKIAA0782 Protein, ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 Protein, centaurin, delta 2 Protein, ARAP1 Protein, centd2 Protein, Arap1 Protein

Sujet: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 (Centaurin-delta-2) (Cnt-d2),FUNCTION: Phosphatidylinositol 3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5 (By similarity). {ECO:0000250}.

Poids moléculaire: 162.3 kDa

UniProt: Q4LDD4