ARHGAP44 Protein (AA 1-814) (Strep Tag)

N° du produit ABIN3135224

Détail du produit

Antigène: ARHGAP44 (rho GTPase Activating Protein 44 (ARHGAP44))

Type de proteíne: Recombinant

Attributs du protein: AA 1-814

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette ARHGAP44 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Séquence: MKKQFNRMRQ LANQTVGRAE KTEVLSEDLL QVEKRLELVK QVSHSTHKKL TACLQGQQGA EADKRSKKLP LTTLAQCLVE GSAILGDDTL LGKMLKLCGE TEDKLAQELI HFELQVERDV IEPLFLLAEV EIPNIQKQRK HLAKLVLDMD SSRTRWQQTS KSSGLSSSLQ PAGAKADALR EEMEEAANRV EICRDQLSAD MYSFVAKEID YANYFQTLIE VQAEYHRKSL TLLQAVLPQI KAQQEAWVEK PSFGKPLEEH LMISGREIAF PIEACVTMLL ECGMQEEGLF RVAPSASKLK KLKAALDCCV VDVQEYSADP HAIAGALKSY LRELPEPLMT FELYDEWIQA SNIQEQDKRL QALWNACEKL PKANHNNIKY LIKFLSKLSE YQDVNKMTPS NMAIVLGPNL LWPQSEGNIT EMMTTVSLQI VGIIEPIIQH ADWFFPGEIE FNLTGSYGSP VHVNHNANYS SMPSPDMDPA DRRQPEQARR PLSVATDNMM LEFYKKDGLR KIQSMGVRVM DTSWVARRGS SAGRKASCAP PSMQPPAPPS ELAAPLPSPL PEQVPDSPAT PAPALSPSGA SLQPTPERPS VSKSKELSPG SGQKGSPGSI QGTPCPGTQL GPQPAASPSQ LPADQSPHTL RKVSKKVAPI PPKVPFVQPG TVSDQPVGQP SPVSLSPTPP STPSPYGLSY PPGYSMASGQ LSPASAPPLA SPSVFTSTLA KSRPTPKPRQ RPTLPPPQPP SVSLSASSPQ STEHPMLDGM SPGESMSTDL VHFDVPSIHI ELGSTLRLSP LEHARRHSAT DKRDSEEESE STAL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ARHGAP44 (rho GTPase Activating Protein 44 (ARHGAP44))

Autre désignation: Arhgap44 (ARHGAP44 Produits)

Synonymes: RICH2 Protein, arhgap44 Protein, NPC-A-10 Protein, 6330543G20 Protein, AI840762 Protein, AU040829 Protein, AW493732 Protein, Rich2 Protein, Rho GTPase activating protein 44 Protein, si:ch211-114m9.1 Protein, ARHGAP44 Protein, si:ch211-114m9.1 Protein, Arhgap44 Protein

Sujet: Rho GTPase-activating protein 44 (Rho-type GTPase-activating protein RICH2) (RhoGAP interacting with CIP4 homologs protein 2) (RICH-2),FUNCTION: GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. Acts as a GAP at least for CDC42 and RAC1 (PubMed:24352656, PubMed:26969129). In neurons, is involved in dendritic spine formation and synaptic plasticity in a specific RAC1-GAP activity (PubMed:23739967, PubMed:24352656, PubMed:26969129). Limits the initiation of exploratory dendritic filopodia. Recruited to actin-patches that seed filopodia, binds specifically to plasma membrane sections that are deformed inward by acto-myosin mediated contractile forces. Acts through GAP activity on RAC1 to reduce actin polymerization necessary for filopodia formation (By similarity). In association with SHANK3, promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation (PubMed:23739967). {ECO:0000250|UniProtKB:F1LQX4, ECO:0000269|PubMed:23739967, ECO:0000269|PubMed:24352656, ECO:0000269|PubMed:26969129}.

Poids moléculaire: 89.0 kDa

UniProt: Q5SSM3