WWC1 Protein (AA 1-1104) (Strep Tag)

N° du produit ABIN3135241

Détail du produit

Antigène: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1104

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette WWC1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MPRPELPLPE GWEEARDFDG KVYYIDHRNR TTSWIDPRDR YTKPLTFADC ISDELPLGWE EAYDPQVGDY FIDHNTKTTQ IEDPRVQWRR EQEHMLKDYL VVAQEALSAQ KEIYQVKQQR LELAQQEYQQ LHAVWEHKLG SQVSLVSGSS SSSKYDPEIL KAEIATAKSR VNKLKREMVH LQHELQFKER GFQTLKKIDE RMSDAQGGYK LDEAQAVLRE TKAIKKAITC GEKEKQDLIK SLAMLKDGFR TDRGSHSDLW SSSSSLESSS FPMPKQFLDV SSQTDISGSF STSSNNQLAE KVRLRLRYEE AKRRIANLKI QLAKLDSEAW PGVLDSERDR LILINEKEEL LKEMRFISPR KWTQGEVEQL EMARRRLEKD LQAARDTQSK ALTERLKLNS KRNQLVRELE EATRQVATLH SQLKSLSSSM QSLSSGSSPG SLTSSRGSLA ASSLDSSTSA SFTDLYYDPF EQLDSELQSK VELLFLEGAT GFRPSGCITT IHEDEVAKTQ KAEGGSRLQA LRSLSGTPRS MTSLSPRSSL SSPSPPCSPL ITDPLLTGDA FLAPLEFEDT ELSTTLCELN LGGSGTQERY RLEEPGPEGK PLGQAASVAP GCGLKVACVS AAVSDESVAG DSGVYEASAQ RPGTSEAAAF DSDESEAVGA TRVQIALKYD EKNKQFAILI IQLSHLSALS LQQDQKVNIR VAILPCSESS TCLFRTRPLD SANTLVFNEA FWVSISYPAL HQKTLRVDVC TTDRSHTEEC LGGAQISLAE VCRSGERSTR WYNLLSYKYL KKQCREPQPT EAPGPDHVDA VSALLEQTAV ELEKRQEGRS SSQTLEGSWT YEEEASENEA VAEEEEEGEE DVFTEKVSPE AEECPALKVD RETNTDSVAP SPTVVRPKDR RVGAPSTGPF LRGNTIIRSK TFSPGPQSQY VCRLNRSDSD SSTLSKKPPF VRNSLERRSV RMKRPSSVKS LRTERLIRTS LDLELDLQAT RTWHSQLTQE ISVLKELKEH LEQAKNHGEK ELPQWLREDE RFRLLLRMLE KKVDRGEHKS ELQADKMMRA AAKDVHRLRG QSCKEPPEVQ SFREKMAFFT RPRMNIPALS ADDV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Autre désignation: Wwc1 (WWC1 Produits)

Synonymes: CG12600 Protein, CG33967 Protein, CG7552 Protein, Dmel\\CG33967 Protein, Kibra Protein, ORE-2 Protein, RE26350 Protein, kib Protein, l(3)02404 Protein, l(3)j5C3 Protein, l(3)neo42 Protein, HBEBP3 Protein, HBEBP36 Protein, KIBRA Protein, AA408228 Protein, AU017197 Protein, BC037006 Protein, mKIAA0869 Protein, CG33967 gene product from transcript CG33967-RB Protein, WW and C2 domain containing 1 Protein, WW, C2 and coiled-coil domain containing 1 Protein, kibra Protein, WWC1 Protein, Wwc1 Protein

Sujet: Protein KIBRA (Kidney and brain protein) (KIBRA) (WW domain-containing protein 1),FUNCTION: Negative regulator of the Hippo signaling pathway, also known as the Salvador-Warts-Hippo (SWH) pathway. Enhances phosphorylation of LATS1 and YAP1 and negatively regulates cell proliferation and organ growth due to a suppression of the transcriptional activity of YAP1, the major effector of the Hippo pathway. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and function in the regulation of Hippo signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Modulates directional migration of podocytes. May be associated with memory performance (By similarity). Regulates collagen-stimulated activation of the ERK/MAPK cascade (PubMed:18190796). Plays an important role in regulating AMPA-selective glutamate receptors (AMPARs) trafficking (PubMed:31730661, PubMed:21943600). {ECO:0000250|UniProtKB:Q8IX03, ECO:0000269|PubMed:18190796, ECO:0000269|PubMed:21943600, ECO:0000269|PubMed:31730661}.

Poids moléculaire: 124.1 kDa

UniProt: Q5SXA9