HNRNPD/AUF1 Protein (AA 2-355) (His tag)

N° du produit ABIN3135276

Détail du produit

Antigène: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Type de proteíne: Recombinant

Attributs du protein: AA 2-355

Origine: Souris

Source: Escherichia coli (E. coli)

Purification/Conjugué: Cette HNRNPD/AUF1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)

Séquence: SEEQFGGDGA AAAATAAVGG SAGEQEGAMV AAAAQGPAAA AGSGSGGGGS AAGGTEGGSA EAEGAKIDAS KNEEDEGHSN SSPRHTEAAA AQREEWKMFI GGLSWDTTKK DLKDYFSKFG EVVDCTLKLD PITGRSRGFG FVLFKESESV DKVMDQKEHK LNGKVIDPKR AKAMKTKEPV KKIFVGGLSP DTPEEKIREY FGGFGEVESI ELPMDNKTNK RRGFCFITFK EEEPVKKIME KKYHNVGLSK CEIKVAMSKE QYQQQQQWGS RGGFAGRARG RGGGPSQNWN QGYSNYWNQG YGNYGYNSQG YGGYGGYDYT GYNNYYGYGD YSNQQSGYGK VSRRGGHQNS YKPY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Hnrnpd Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Autre désignation: Hnrnpd (HNRNPD Produits)

Synonymes: AUF1 Protein, AUF1A Protein, HNRPD Protein, P37 Protein, hnRNPD0 Protein, Auf1 Protein, Hnrnpd Protein, HNRNPD Protein, p37 Protein, auf1a Protein, hnrpd Protein, hnrnpd0 Protein, C230004L04 Protein, Hnrpd Protein, wu:fa28b06 Protein, zgc:162951 Protein, heterogeneous nuclear ribonucleoprotein D Protein, heterogeneous nuclear ribonucleoprotein D L homeolog Protein, HNRNPD Protein, Hnrnpd Protein, hnrnpd Protein, hnrnpd.L Protein

Sujet: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. Binding of RRM1 to DNA inhibits the formation of DNA quadruplex structure which may play a role in telomere elongation. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. May play a role in the regulation of the rhythmic expression of circadian clock core genes. Directly binds to the 3'UTR of CRY1 mRNA and induces CRY1 rhythmic translation. May also be involved in the regulation of PER2 translation. {ECO:0000250|UniProtKB:Q14103}.

Poids moléculaire: 39.2 kDa Including tag.

UniProt: Q60668