PP5 Protein (AA 2-499) (His tag)

N° du produit ABIN3135282

Détail du produit

Antigène: PP5 (PPP5C) (Protein Phosphatase 5, Catalytic Subunit (PPP5C))

Type de proteíne: Recombinant

Attributs du protein: AA 2-499

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette PP5 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: AMAEGERTEC AETPRDEPPA DGTLKRAEEL KTQANDYFKA KDYENAIKFY SQAIELNPGN AIYYGNRSLA YLRTECYGYA LGDATRAIEL DKKYIKGYYR RAASNMALGK FRAALRDYET VVKVKPNDKD AKMKYQECSK IVKQKAFERA IAGDEHRRSV VDSLDIESMT IEDEYSGPKL EDGKVTITFM KDLMQWYKDQ KKLHRKCAYQ ILVQVKEVLC KLSTLVETTL KETEKITVCG DTHGQFYDLL NIFELNGLPS ETNPYIFNGD FVDRGSFSVE VILTLFGFKL LYPDHFHLLR GNHETDNMNQ IYGFEGEVKA KYTAQMYELF SEVFEWLPLA QCINGKVLIM HGGLFSEDGV TLDDIRKIER NRQPPDSGPM CDLLWSDPQP QNGRSVSKRG VSCQFGPDVT KAFLEENQLD YIIRSHEVKA EGYEVAHGGR CVTVFSAPNY CDQMGNKASY IHLQGSDLRP QFHQFTAVPH PNVKPMAYAN TLLQLGMM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ppp5c Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PP5 (PPP5C) (Protein Phosphatase 5, Catalytic Subunit (PPP5C))

Autre désignation: Ppp5c (PPP5C Produits)

Synonymes: PP5 Protein, PPP5 Protein, PPT Protein, AU020526 Protein, pp5 Protein, MGC89961 Protein, fc83f08 Protein, im:7146608 Protein, wu:fc83f08 Protein, zgc:110801 Protein, PPP5C Protein, protein phosphatase 5 catalytic subunit Protein, protein phosphatase 5, catalytic subunit Protein, protein phosphatase 5, catalytic subunit S homeolog Protein, serine/threonine-protein phosphatase 5 Protein, PPP5C Protein, Ppp5c Protein, ppp5c.S Protein, ppp5c Protein, LOC592155 Protein, LOC100513542 Protein, LOC100564151 Protein, LOC100186268 Protein, LOC100208775 Protein

Sujet: Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. Also dephosphorylates and inhibits the anti-adipogenic effect of NR3C1. Regulates the circadian rhythms, through the dephosphorylation and activation of CSNK1E. May modulate TGF-beta signaling pathway by the regulation of SMAD3 phosphorylation and protein expression levels. Dephosphorylates and may play a role in the regulation of TAU/MAPT. Through their dephosphorylation, may play a role in the regulation of ions channels such as KCNH2. {ECO:0000269|PubMed:17376776, ECO:0000269|PubMed:21994940, ECO:0000269|PubMed:22526606}.

Poids moléculaire: 57.7 kDa Including tag.

UniProt: Q60676