SIX4 Protein (AA 2-775) (His tag)

N° du produit ABIN3135401

Détail du produit

Antigène: SIX4 (SIX Homeobox 4 (SIX4))

Type de proteíne: Recombinant

Attributs du protein: AA 2-775

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette SIX4 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: SSSSPTGQIA SAADIKQENG MESASEGQEA HREVAGGAAA GLSPPAPAPF PLEPGDAAAA SRVSREEGAA AAGAADQVQL HSELLGRHQH AAAAQPPLAF SPDHVACVCE ALQQGGNLDR LARFLWSLPQ SDLLRGNESL LKARALVAFH QGIYPELYSI LESHSFESAN HPLLQQLWYK ARYTEAERAR GRPLGAVDKY RLRRKFPLPR TIWDGEETVY CFKEKSRNAL KELYKQNRYP SPAEKRHLAK ITGLSLTQVS NWFKNRRQRD RNPSETQSKS ESDGNPSTED ESSKGHEDLS PHPLSGASDG VTNLSLSSHV EPVYMQQIGN AKISLSSSGV LLNGSLVPAS TSPVFLNGNS FIQGHNGVIL NGLNVGNTQT VSLNPPKMSS NIVGNGIAMT DILGSTSQDV KEFKVLQSSA VNSAATTSYS PSAPVSFPGL IPCTEVKREG IQTVASQDGG SVVTFTTPVQ INQYGIVQIP NSGANGQFLN GSIGFSPLQL PPVSVAASQG NLSVTPSTSD GSTFTSEPAT VQHGKLFLSP LTPSAVVYTV PNSGQTVGAV KQEGLERGLV FSQLMPVNHS AQVNASLSSE NLSGSGLHPL TSSLVNVSAA HGFSLTPPTL LNPTELNPDL AESQPVSAPV ASKCTVSSVS NTNYATLQNC SLIPGQDLLS GPMTQAALGE IVPTAEEQVS HASTAVHQDF VREQRLVLQS VPNIKENFLQ NSENKATNNL MMLDSKSKYV LDGMVEAGCE DLGTDKKELA KLQTVQLDED MQDL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Six4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SIX4 (SIX Homeobox 4 (SIX4))

Autre désignation: Six4 (SIX4 Produits)

Synonymes: AREC3 Protein, AI047561 Protein, TrexBF Protein, fk50f02 Protein, six4.2 Protein, six4b Protein, wu:fk50f02 Protein, SIX homeobox 4 Protein, sine oculis-related homeobox 4 Protein, SIX homeobox 4a Protein, SIX4 Protein, Six4 Protein, six4a Protein

Sujet: Transcriptional regulator which can act as both a transcriptional repressor and activator by binding a DNA sequence on these target genes and is involved in processes like cell differentiation, cell migration and cell survival. Transactivates gene expression by binding a 5'-[CAT]A[CT][CT][CTG]GA[GAT]-3' motif present in the Trex site and from a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 site of the muscle-specific genes enhancer (PubMed:14966291). Acts cooperatively with EYA proteins to transactivate their target genes through interaction and nuclear translocation of EYA protein (PubMed:10490620). Acts synergistically with SIX1 to regulate target genes involved in formation of various organs, including muscle, kidney, gonad, ganglia, olfactory epithelium and cranial skeleton. Plays a role in several important steps of muscle development. Controls the genesis of hypaxial myogenic progenitors in the dermomyotome by transactivating PAX3 and the delamination and migration of the hypaxial precursors from the ventral lip to the limb buds through the transactivation of PAX3, MET and LBX1 (PubMed:15788460). Controls myoblast determination by transactivating MYF5, MYOD1 and MYF6 (PubMed:15788460, PubMed:17592144). Controls somitic differentiation in myocyte through MYOG transactivation (PubMed:15788460). Plays a role in synaptogenesis and sarcomere organization by participating in myofiber specialization during embryogenesis by activating fast muscle program in the primary myotome resulting in an up-regulation of fast muscle genes, including ATP2A1, MYL1 and TNNT3 (PubMed:19962975, PubMed:21884692). Simultaneously, is also able to activate inhibitors of slow muscle genes, such as SOX6, HRASLS, and HDAC4, thereby restricting the activation of the slow muscle genes (PubMed:21884692). During muscle regeneration, negatively regulates differentiation of muscle satellite cells through down-regulation of MYOG expression (PubMed:20696153). During kidney development regulates the early stages of metanephros development and ureteric bud formation through regulation of GDNF, SALL1, PAX8 and PAX2 expression (PubMed:17300925). Plays a role in gonad development by regulating both testis determination and size determination. In gonadal sex determination, transactivates ZFPM2 by binding a MEF3 consensus sequence, resulting in SRY up-regulation. In gonadal size determination, transactivates NR5A1 by binding a MEF3 consensus sequence resulting in gonadal precursor cell formation regulation (PubMed:23987514). During olfactory development mediates the specification and patterning of olfactory placode through fibroblast growth factor and BMP4 signaling pathways and also regulates epithelial cell proliferation during placode formation (PubMed:19027001). Promotes survival of sensory neurons during early trigeminal gangliogenesis (PubMed:16938278). In the developing dorsal root ganglia, up-regulates SLC12A2 transcription (PubMed:15955062). Regulates early thymus/parathyroid organogenesis through regulation of GCM2 and FOXN1 expression (PubMed:16530750). Forms gustatory papillae during development of the tongue (PubMed:21978088). Also plays a role during embryonic cranial skeleton morphogenesis (PubMed:20515681). {ECO:0000269|PubMed:10490620, ECO:0000269|PubMed:14966291, ECO:0000269|PubMed:15788460, ECO:0000269|PubMed:15955062, ECO:0000269|PubMed:16530750, ECO:0000269|PubMed:16938278, ECO:0000269|PubMed:17300925, ECO:0000269|PubMed:17592144, ECO:0000269|PubMed:19027001, ECO:0000269|PubMed:19962975, ECO:0000269|PubMed:20515681, ECO:0000269|PubMed:20696153, ECO:0000269|PubMed:21884692, ECO:0000269|PubMed:21978088, ECO:0000269|PubMed:23987514, ECO:0000303|PubMed:10490620}.

Poids moléculaire: 83.1 kDa Including tag.

UniProt: Q61321

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development