STK36 Protein (AA 1-1316) (Strep Tag)

N° du produit ABIN3135657

Détail du produit

Antigène: STK36 (serine/threonine Kinase 36 (STK36))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1316

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette STK36 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEKYHVLEMI GEGSFGRVYK GRKKYSAQVV ALKFIPKLGR SEKELRNLQR EIEIMRGLWH PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED QVQAIAAQLV SALYYLHSHR ILHRDMKPQN ILLAKGGGIK LCDFGFARAM STNTMVLTSI KGTPLYMSPE LVEERPYDHT ADLWSVGCIL YELAVGTPPF YTTSIFQLVS LILKDPVRWP STISSCFKNF LQGLLTKDPR QRLSWPDLLH HPFIAGRVTI ITEPAGSDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR ILRQACKLMA EEAKQKEDQN AGSALEQEDG LCKVTPSTAP VPGLKATPQE SSLLAGILAS EMKNNWEDWG AGEAPRTSRE NHINLECEQG FPEPRPEAMG RQSTDVVDPE NEEPDSDDEW QRLLETSEPG PVQLKSPLTL LCNPDFCQRI QSQLRGTGEQ ILKGVLDGVS HLLPVLRILS SLLSSCNDSV LLYSFCQEAG LPELPLSLLR YSQESSSIQQ QPWYGALLRD LVAVVQAYFS CTFNLERSQT GDSLQVFQEA ASLFLDLLGK LLAQSDDSEQ TFRRDSLMCF AVLCEAVDGN SWAVSKAFYS SLLTTQRAVL DGLLHGLTVP QLPFHTPPGA PQVSQPLREQ SEDVPGAISS ALAAMCTAPV GLPSCWDAKE QVSWHLANQL TEDSSQLRPS LISGLRHHVL CLHLLKVLYA CCYISERLCH ILGQEPLALE SLLMLVQGKV KVADWEESTE VALYLLSLLV FRLQDLPSGM EKLGSEVATL FTHSHVVSLV NAAACLLGQL GQQGVTFDLQ PREWIAAAAH ALSAPAEVRL TPPYSCGFYD GLLILLLQLL MQVQGKPGLI RDVVGSEVWT ILWHRFSMAL RLPEEVSAQE DDLLLSSPSS LEPDWTLISP QGMAALLSLA MAIFTQESQL CLSHLSQHGS VLMLTLKHLL SPSFLHHLSQ APQGPEFLPV VVLSVCKLLC FPFALDVDAD LLVGVLADLR ASEVVVCLLQ VCCHHLSLLQ AELPIGLLTR LALTDSASLK QFVNTVATSS RAIISFLSVV LLSDQPLMIS DLLSLLTHTA RILSPSHLSF IQELLSGSDE SYRPLRSLLG HSENTVRVRA YGLLGHLLQH SMALRGALQS QSGLLNLLLL GLGDKDPAVR RSASFAVGNA AYQAGPLGPA LAAAVPSMTQ LLGDAQDGIR RNAASALGNL GPEGLGKELL KCQVPQRLLE MACGDPQPTV KEAALIALRS LQQESCIHQV LVSLGASEKL ALLSLGNQLL PNSSNRPASV RHCRKLIQLL RPTHST
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: STK36 (serine/threonine Kinase 36 (STK36))

Autre désignation: Stk36 (STK36 Produits)

Synonymes: FU Protein, fused-like Protein, 1700112N14Rik Protein, B930045J24 Protein, Fused Protein, mKIAA1278 Protein, fu Protein, si:dkey-180i4.3 Protein, zgc:152944 Protein, serine/threonine kinase 36 Protein, serine/threonine kinase 36 (fused homolog, Drosophila) Protein, STK36 Protein, Stk36 Protein, stk36 Protein

Sujet: Serine/threonine-protein kinase 36 (EC 2.7.11.1) (Fused homolog),FUNCTION: Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors. Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur. Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function. Essential for construction of the central pair apparatus of motile cilia. {ECO:0000269|PubMed:16055717, ECO:0000269|PubMed:18600476, ECO:0000269|PubMed:19305393}.

Poids moléculaire: 144.2 kDa

UniProt: Q69ZM6