N4BP1 Protein (AA 1-893) (Strep Tag)

N° du produit ABIN3135669

Détail du produit

Antigène: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-893

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette N4BP1 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MAARVVLDEF TAPAEKAALL ERSRGRIEAL FGVGLAVLGA LGAEEPLPAR IWLQLRGAQE AVHSAKEYIK GICEPELEEK ECYPKAMHCI FVGAQSLFLK SLIQDTCADL CVLDTGLLGI RGSAEAVVMA RSHIQQFVKL FESNENLPSN QRESEIKREF RQFVEAHADS YTMDLLILPT SLKKELLSLT QGEESLFETD DDVITVGDVR PPEYTQSAAT GPSSARDEVV VQEDSRNKAR TPVSELTKHM DTVFSSSPDV LFVPVNGLSP DEDALSKDRV CHKRRSSDTE ERHTKKQFSL ENVPEGELLP DGKGSAGNEV IDLSDPASNS TNLSPDGKDT TEEMEYNILV NFFKTMGYSQ EIVEKVIREY GPSTEPLLLL EEIEKENKRL QEDRDFPPCT VYPDASQSRN AGVGSTTNEL TADSTPKKAQ SHTEQSMVER FSQLPFKDSK HCTSNCKVNS FRTVPVGQKQ EIWGSKQNSS CTVDLETDGH SASAASASPK DISFVSRGAS GHQQRNPAFP ENGFQQQTEP LLPNNTKPAC EKRSGSCSSP QPKPNYPPLS PPLPLPQLLP SVTEARLGGS SDHIDSSVTG VQRFRDTLKI PYKLELKNEP GRADLKHIVI DGSNVAITHG LKKFFSCRGI AIAVEYFWKL GNRNITVFVP QWRTRRDPNI TEQHFLTQLQ ELGILSLTPA RMVFGERIAS HDDRFLLHLA DKTGGIIVTN DNFREFVTES VSWREIITKR LLQYTFVGDI FMVPDDPLGR NGPRLEEFLR KEAFLRHMQP LLNALPSVGT FDPGFRSPST QVANNSHQPP PRIQTSSSPW LPQQSHFTAL ATLPSMQQNP PLPAQRSSAE TSELREALLK IFPDSEQKLK IDQILAAHPY MKDLNALSAL VLD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: N4BP1 (NEDD4 Binding Protein 1 (N4BP1))

Autre désignation: N4bp1 (N4BP1 Produits)

Synonymes: AI481586 Protein, C81621 Protein, RGD1305179 Protein, wu:fi03b05 Protein, zgc:66437 Protein, NEDD4 binding protein 1 Protein, Nedd4 binding protein 1 Protein, nedd4 binding protein 1 Protein, nedd4 binding protein 1 L homeolog Protein, N4BP1 Protein, N4bp1 Protein, n4bp1 Protein, n4bp1.L Protein

Sujet: NEDD4-binding protein 1 (N4BP1) (EC 3.1.-.-),FUNCTION: Potent suppressor of cytokine production that acts as a regulator of innate immune signaling and inflammation (PubMed:32971525). Acts as a key negative regulator of select cytokine and chemokine responses elicited by TRIF-independent Toll-like receptors (TLRs), thereby limiting inflammatory cytokine responses to minor insults (PubMed:32971525). In response to more threatening pathogens, cleaved by CASP8 downstream of TLR3 or TLR4, leading to its inactivation, thereby allowing production of inflammatory cytokines (PubMed:32971525). Acts as a restriction factor against some viruses: restricts viral replication by binding to mRNA viruses and mediating their degradation via its ribonuclease activity (By similarity). Also acts as an inhibitor of the E3 ubiquitin-protein ligase ITCH: acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (PubMed:17592138). {ECO:0000250|UniProtKB:O75113, ECO:0000269|PubMed:17592138, ECO:0000269|PubMed:32971525}.

Poids moléculaire: 99.1 kDa

UniProt: Q6A037