NCAPG2 Protein (AA 1-1138) (Strep Tag)

N° du produit ABIN3135680

Détail du produit

Antigène: NCAPG2 (Non-SMC Condensin II Complex, Subunit G2 (NCAPG2))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1138

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NCAPG2 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEKREAFIQA VSKELVEEFL QFLQLDKDSS NPFSLSELLD ELSRKQKEEL WQRLKDLLTE TLLESPVDRW QTVEVEGADD MESEHSPKMR KSIKIICAIV TVILASVSII NEHENYGALL ECAVILNGIL YALPESEQKL QNSIQDLCVK WWERGLPAKE DMGKTAFIML LRRSLETKSG ADVCRLWRIH QALYCFDYDW EESREIKDML LECFINVNYI KKEEGRRFLS FLFSWNVDFI KMIHETIKNQ LAGLQKSLMV HIAEIYFRAW KKASGKMLET IEYDCIQDFM FHGIHLLRRS PVHSKVREVL SYFHQQKVRQ GVEEMLYRLY KPILWRGLKA RNSEVRSNAA LLFVEAFPIR DPNFTATEMD NEIQKQFEEL YNLIEDPYPR VRSTGILGVC KISSKYWEMM PPNILVDFLK KVTGELAFDI SSADVRCSVF KCLPIILDNK LSHPLLEQLL PTLRYSLHDN SEKVRVAFVD LLLKIKAVRA AKFWKICPME DILVRLEMDS RPVSRRLVSL IFNSFLPVNQ PEEVWCERCV TLIQMNRAAA RRFYQYAHEH TASTNIAKLI HVIRHCLNAC IQRTLREGSE AHKECEKENA SVLDKTLSVN DTASMAGLLE IIVILWKNIH RSLENNKEAK IYTINKFAAV LPEYLKVFKD ERCKIPLFML MSFLPASAVP VFSCGVISVL RNQESVTGRS YCTLLDCLCS WGQVGHVLEL IVDWLPTVPP QAKSNLASKR KVEINDTCSV KPELALLYME YLLTHPKNRE CLLSVPQKKL NQLLKALEGS KAELESFLQS PSGNPLNFNK ATALHAFGLY CRMSVHLQYK FCSEEKIHLS ILDDTGSWLE NKVLPLLEDQ EEEYLKLRKD VYQQIIQTYL AVCKDVVMVG LGDPKFQMQL LQRSFGIMKT VKGFFYVSLL LGILKEIAGN TIIHKTDSDE KVTVLFDLVQ EVFQKMLECI ACIFRKQPEE SLPLFHSVQT PLHEFITTIQ SWHKDTAVHH AVLSTLIAAP VVEISHQLQK VSDIEELTSP QCLHDLPPFS RCLVGVIMKS SDVVRSFVDE LKACVTSGDV EGIVCLTAVL HIILVINKGK HISAKVKEVA TAVYRKLKTF MEITLEEDSL ERFLYESSMR TLGEFLNP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NCAPG2 (Non-SMC Condensin II Complex, Subunit G2 (NCAPG2))

Autre désignation: Ncapg2 (NCAPG2 Produits)

Synonymes: NCAPG2 Protein, LUZP5 Protein, id:ibd2775 Protein, zgc:162881 Protein, FAM62B Protein, CAP-G2 Protein, CAPG2 Protein, MTB Protein, hCAP-G2 Protein, 5830426I05Rik Protein, Luzp5 Protein, Mtb Protein, mCAP-G2 Protein, RGD1308406 Protein, non-SMC condensin II complex, subunit G2 Protein, non-SMC condensin II complex subunit G2 Protein, extended synaptotagmin 2 Protein, condensin-2 complex subunit G2 Protein, non-SMC condensin II complex subunit G2 L homeolog Protein, NCAPG2 Protein, ncapg2 Protein, ESYT2 Protein, LOC100401712 Protein, Ncapg2 Protein, ncapg2.L Protein

Sujet: Condensin-2 complex subunit G2 (Chromosome-associated protein G2) (CAP-G2) (Leucine zipper protein 5) (More than blood protein) (Non-SMC condensin II complex subunit G2),FUNCTION: Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis. Is required for early embryonic development and is essential for viability and expansion of the inner cell mass (ICM) of the implanting blastocyst. {ECO:0000269|PubMed:14729962}.

Poids moléculaire: 130.9 kDa

UniProt: Q6DFV1